ArticleThe Journal of cell biology2000
Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
- Microtubule nucleation for spindle assembly: one molecule at a time.Trends in biochemical sciences · 2023Review
- Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources.iScience · 2023Article
- Regulating Phase Transition in Neurodegenerative Diseases by Nuclear Import Receptors.Biology · 2022Review
- O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport.The Journal of cell biology · 2021Article
- Complementary functions for the Ran gradient during division.Small GTPases · 2021Review
- Ran GTPase: A Key Player in Tumor Progression and Metastasis.Frontiers in cell and developmental biology · 2020Review
- Heterogeneous Nuclear Ribonucleoprotein A1 and Lamin A/C Modulate Nucleocytoplasmic Shuttling of Avian Reovirus p17.Journal of virology · 2019Article
- Three-dimensional context rather than NLS amino acid sequence determines importin α subtype specificity for RCC1.Nature communications · 2017Article
- Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.Molecular biology of the cell · 2016Article
- Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport.Molecular biology of the cell · 2016Article
- The Ran Pathway in Drosophila melanogaster Mitosis.Frontiers in cell and developmental biology · 2015Review
- Solution structures of Mengovirus Leader protein, its phosphorylated derivatives, and in complex with nuclear transport regulatory protein, RanGTPase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Cell cycle-dependent binding modes of the ran exchange factor RCC1 to chromatin.Biophysical journal · 2013Article
- Spatial cycles in G-protein crowd control.The EMBO journal · 2010Review
- The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope.Molecular and cellular biology · 2010Article
- The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells.BMC cell biology · 2010Article
- Organization and regulation of nucleocytoplasmic transport.Molecular biology · 2010Review
- Mechanisms and signals for the nuclear import of proteins.Current genomics · 2009Article
- Avian reovirus sigmaA localizes to the nucleolus and enters the nucleus by a nonclassical energy- and carrier-independent pathway.Journal of virology · 2009Article
- Functional analysis of key nuclear trafficking components reveals an atypical Ran network required for parasite pathogenesis.Molecular microbiology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RCC1, the only known guanine-nucleotide exchange factor for the Ran GTPase, is an approximately 45-kD nuclear protein that can bind chromatin. An important question concerns how RCC1 traverses the nuclear envelope. We now show that nuclear RCC1 is not exported readily in interphase cells and that the import of RCC1 into the nucleoplasm is extremely rapid. Import can proceed by at least two distinct mechanisms. The first is a classic import pathway mediated by basic residues within the NH(2)-terminal domain (NTD) of RCC1. This pathway is dependent upon both a preexisting Ran gradient and energy, and preferentially uses the importin-alpha3 isoform of importin-alpha. The second pathway is not mediated by the NTD of RCC1. This novel pathway does not require importin-alpha or importin-beta or the addition of any other soluble factor in vitro; however, this pathway is saturable and sensitive only to a subset of inhibitors of classical import pathways. Furthermore, the nuclear import of RCC1 does not require a preexisting Ran gradient or energy. We speculate that this second import pathway evolved to ensure that RCC1 never accumulates in the cytoplasm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.