ArticleThe EMBO journal2000
Thr38 and Ser198 are Pto autophosphorylation sites required for the AvrPto-Pto-mediated hypersensitive response.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 104 citations in OpenAlex.
- Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.PLoS pathogens · 2021Article
- Phosphorylation regulates the subcellular localization of Cucumber Mosaic Virus 2b protein.Scientific reports · 2017Article
- Evidence-based gene models for structural and functional annotations of the oil palm genome.Biology direct · 2017Article
- Identification of critical functional residues of receptor-like kinase ERECTA.Journal of experimental botany · 2017Article
- Genome-Wide Analysis and Evolution of the Pto-Like Protein Kinase (PLPK) Gene Family in Pepper.PloS one · 2016Article
- Transcriptional dynamics of Phytophthora infestans during sequential stages of hemibiotrophic infection of tomato.Molecular plant pathology · 2016Article
- Analysis of Phosphorylation of the Receptor-Like Protein Kinase HAESA during Arabidopsis Floral Abscission.PloS one · 2016Article
- Kinase domain-targeted isolation of defense-related receptor-like kinases (RLK/Pelle) in Platanus×acerifolia: phylogenetic and structural analysis.BMC research notes · 2014Article
- Identification of post-translational modifications of plant protein complexes.Journal of visualized experiments : JoVE · 2014Article
- The tomato Prf complex is a molecular trap for bacterial effectors based on Pto transphosphorylation.PLoS pathogens · 2013Article
- Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.PloS one · 2013Article
- The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses.BMC genomics · 2012Article
- Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Article
- Biochemical and genetic requirements for function of the immune response regulator BOTRYTIS-INDUCED KINASE1 in plant growth, ethylene signaling, and PAMP-triggered immunity in Arabidopsis.The Plant cell · 2011Article
- Crystal structure of the complex between Pseudomonas effector AvrPtoB and the tomato Pto kinase reveals both a shared and a unique interface compared with AvrPto-Pto.The Plant cell · 2009Article
- Bypassing kinase activity of the tomato Pto resistance protein with small molecule ligands.The Journal of biological chemistry · 2009Article
- A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance.Plant molecular biology · 2009Article
- Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity.PLoS biology · 2008Article
- Genetic diversity of Pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries.Journal of molecular evolution · 2008Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tomato Pto kinase confers resistance to Pseudomonas syringae pv. tomato expressing the AvrPto protein. To elucidate the role of Pto autophosphorylation in disease resistance, eight sites autophosphorylated by Pto in vitro were identified by a combination of HPLC purification of tryptic phosphopeptides, MALDI-TOF/MS analysis and Edman degradation. Mutational analysis of the autophosphorylation sites revealed that Pto residues Thr38 and Ser198 are required for AvrPto-Pto- mediated elicitation of a hypersensitive response in the plant. Thr38, which is the main Pto autophosporylation site and is located outside the kinase catalytic domain, was also required for Pto kinase activity and its physical interaction with AvrPto, the Pti1 kinase and the transcription factor Pti4. Ser198, located in the Pto activation domain, was dispensable for kinase activity and for interaction with AvrPto. However, a mutation at this site resulted in altered Pto interactions with the Pti1 kinase and the Pto interactors of unknown function Pti3 and Pti10. These results suggest that autophosphorylation events at Pto Thr38 and Ser198 are required for signal transduction by Pto and participate in distinct molecular mechanisms.
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