ArticleThe EMBO journal2000
A temperature-sensitive adenylyl cyclase mutant of Dictyostelium.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP.Biology open · 2023Article
- Oscillatory cAMP cell-cell signalling persists during multicellularCommunications biology · 2019Article
- Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Progress and perspectives in signal transduction, actin dynamics, and movement at the cell and tissue level: lessons fromInterface focus · 2016Review
- Self-organization of chemoattractant waves in Dictyostelium depends on F-actin and cell-substrate adhesion.Journal of the Royal Society, Interface · 2016Article
- The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.PloS one · 2013Article
- Imaging of cell migration.The EMBO journal · 2006Review
- The cyclase-associated protein CAP as regulator of cell polarity and cAMP signaling in Dictyostelium.Molecular biology of the cell · 2004Article
- Becoming Multicellular by Aggregation; The Morphogenesis of the Social Amoebae Dicyostelium discoideum.Journal of biological physics · 2002Article
- A Dictyostelium nuclear phosphatidylinositol phosphate kinase required for developmental gene expression.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Dictyostelium development starts with the chemotactic aggregation of up to 10(6) amoebae in response to propagating cAMP waves. cAMP is produced by the aggregation stage adenylyl cyclase (ACA) and cells lacking ACA (aca null) cannot aggregate. Temperature-sensitive mutants of ACA were selected from a population of aca null cells transformed with a library of ACA genes, a major segment of which had been amplified by error-prone PCR. One mutant (tsaca2) that can complement the aggregation null phenotype of aca null cells at 22 degrees C but not at 28 degrees C was characterized in detail. The basal catalytic activity of the enzyme in this mutant was rapidly and reversibly inactivated at 28 degrees C. Using this mutant strain we show that cell movement in aggregates and mounds is organized by propagating waves of cAMP. Synergy experiments between wild-type and tsaca2 cells, shifted to the restrictive temperature at various stages of development, showed that ACA plays an important role in the control of cell sorting and tip formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.