ArticleGenome research2000
Comparative genomic sequencing identifies novel tissue-specific enhancers and sequence elements for methylation-sensitive factors implicated in Igf2/H19 imprinting.
Article in Genome research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- ASHIC: hierarchical Bayesian modeling of diploid chromatin contacts and structures.Nucleic acids research · 2020Article
- Profound alterations of the chromatin architecture at chromosome 11p15.5 in cells from Beckwith-Wiedemann and Silver-Russell syndromes patients.Scientific reports · 2020Article
- The insulin-like growth factor 2 gene in mammals: Organizational complexity within a conserved locus.PloS one · 2019Article
- A Japanese history of the Human Genome Project.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2019Article
- The insulin-like growth factor 2 gene and locus in nonmammalian vertebrates: Organizational simplicity with duplication but limited divergence in fish.The Journal of biological chemistry · 2018Article
- Similarity and variation in the insulin-like growth factor 2 - H19 locus in primates.Physiological genomics · 2018Article
- The complex genetics of human insulin-like growth factor 2 are not reflected in public databases.The Journal of biological chemistry · 2018Article
- Paxillin-dependent regulation of IGF2 and H19 gene cluster expression.Journal of cell science · 2015Article
- Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta.Human molecular genetics · 2014Article
- The Igf2/H19 muscle enhancer is an active transcriptional complex.Nucleic acids research · 2013Article
- Induced DNA demethylation can reshape chromatin topology at the IGF2-H19 locus.Nucleic acids research · 2013Article
- Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse.Development (Cambridge, England) · 2013Article
- Promoter cross-talk via a shared enhancer explains paternally biased expression of Nctc1 at the Igf2/H19/Nctc1 imprinted locus.Nucleic acids research · 2013Article
- Long-range chromatin interactions at the mouse Igf2/H19 locus reveal a novel paternally expressed long non-coding RNA.Nucleic acids research · 2011Article
- Long range interactions regulate Igf2 gene transcription during skeletal muscle differentiation.The Journal of biological chemistry · 2010Article
- Imprinted genes show unique patterns of sequence conservation.BMC genomics · 2010Article
- Cohesin is required for higher-order chromatin conformation at the imprinted IGF2-H19 locus.PLoS genetics · 2009Article
- A novel H19 antisense RNA overexpressed in breast cancer contributes to paternal IGF2 expression.Molecular and cellular biology · 2008Article
- Expression of a modified H19 RNA does not cause embryonic lethality in mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Article
- Rasgrf1 imprinting is regulated by a CTCF-dependent methylation-sensitive enhancer blocker.Molecular and cellular biology · 2005Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A differentially methylated region (DMR) and endoderm-specific enhancers, located upstream and downstream of the mouse H19 gene, respectively, are known to be essential for the reciprocal imprinting of Igf2 and H19. To explain the same imprinting patterns in non-endodermal tissues, additional enhancers have been hypothesized. We determined and compared the sequences of human and mouse H19 over 40 kb and identified 10 evolutionarily conserved downstream segments, 2 of which were coincident with the known enhancers. Reporter assays in transgenic mice showed that 5 of the other 8 segments functioned as enhancers in specific mesodermal and/or ectodermal tissues. We also identified a conserved 39-bp element that appeared repeatedly within the DMR and formed complexes with specific nuclear factors. Binding of one of the factors was inhibited when the target sequence contained methylated CpGs. These complexes may contribute to the presumed boundary function of the unmethylated DMR, which is proposed to insulate maternal Igf2 from the enhancers. Our results demonstrate that comparative genomic sequencing is highly efficient in identifying regulatory elements.
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