ArticleJournal of bacteriology2000
Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 169 citations in OpenAlex.
- Microbial chitinases in the plant holobiont: ecological roles and structural determinants.FEMS microbiology reviews · 2026Review
- Distinct cellular and molecular mechanisms contribute to the specificity of the two Drosophila melanogaster chitin synthases in chitin deposition.PLoS genetics · 2025Article
- Article
- Role of Natural Binding Proteins in Therapy and Diagnostics.Life (Basel, Switzerland) · 2024Article
- In silico approaches for the identification of potential allergens among hypothetical proteins from Alternaria alternata and its functional annotation.Scientific reports · 2024Article
- Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine.Applied microbiology and biotechnology · 2023Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- Expression, purification, and basic properties of a novel domain structure possessing chitinase from Escherichia coli carrying the family 18 chitinase gene of Bacillus velezensis strain RB.IBE29.Molecular biology reports · 2022Article
- Functional amyloid-chitin hybrid ink coupled with flexible fabrication approaches for diverse macro and micro-structures.Materials today. Bio · 2022Article
- Chitinase Chit62J4 Essential for Chitin Processing by Human Microbiome BacteriumMolecules (Basel, Switzerland) · 2021Article
- Fabrication of silica on chitin in ambient conditions using silicatein fused with a chitin-binding domain.Bioprocess and biosystems engineering · 2021Article
- Nanoparticles With Affinity for α-Synuclein Sequester α-Synuclein to Form Toxic Aggregates in Neurons With Endolysosomal Impairment.Frontiers in molecular neuroscience · 2021Article
- Chitinase Gene Positively Regulates Hypersensitive and Defense Responses of Pepper toInternational journal of molecular sciences · 2020Article
- Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC biotechnology · 2020Article
- Enzymatic characterization and structure-function relationship of two chitinases, LmChiA and LmChiB, fromHeliyon · 2020Article
- AToxins · 2020Article
- Chitooligosaccharides and Thermostable Chitinase Against Vulvovaginal Candidiasis and Saprophyte Fungi: LC Mass Studies of Shrimp Shell Fermentation by Bacillus altitudinis.Current microbiology · 2020Article
- Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB FromFrontiers in bioengineering and biotechnology · 2020Article
- Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization.Frontiers in bioengineering and biotechnology · 2020Article
- Accelerated CO₂ Hydration with ThermostableInternational journal of molecular sciences · 2019Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBD). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) specifically bound to various forms of insoluble chitin but not to other polysaccharides, including chitosan, cellulose, and starch. Interaction of soluble chitinous substrates with ChBD(ChiA1) was not detected by means of nuclear magnetic resonance and isothermal titration calorimetry. In addition, the presence of soluble substrates did not interfere with the binding of ChBD(ChiA1) to regenerated chitin. These observations suggest that ChBD(ChiA1) recognizes a structure which is present in insoluble or crystalline chitin but not in chito-oligosaccharides or in soluble derivatives of chitin. ChBD(ChiA1) exhibited binding activity over a wide range of pHs, and the binding activity was enhanced at pHs near its pI and by the presence of NaCl, suggesting that the binding of ChBD(ChiA1) is mediated mainly by hydrophobic interactions. Hydrolysis of beta-chitin microcrystals by intact chitinase A1 and by a deletion derivative lacking the ChBD suggested that the ChBD is not absolutely required for hydrolysis of beta-chitin microcrystals but greatly enhances the efficiency of degradation.
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