ArticleJournal of bacteriology2000
Identification of an effector specificity subregion within the aromatic-responsive regulators DmpR and XylR by DNA shuffling.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors.ACS synthetic biology · 2025Review
- The predicted σ(54)-dependent regulator EtpR is essential for expression of genes for anaerobic p-ethylphenol and p-hydroxyacetophenone degradation in "Aromatoleum aromaticum" EbN1.BMC microbiology · 2015Article
- Transcription factor-based biosensors enlightened by the analyte.Frontiers in microbiology · 2015Review
- Article
- The methanogen-specific transcription factor MsvR regulates the fpaA-rlp-rub oxidative stress operon adjacent to msvR in Methanothermobacter thermautotrophicus.Journal of bacteriology · 2010Article
- Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator.BMC microbiology · 2008Article
- Tracing explosives in soil with transcriptional regulators of Pseudomonas putida evolved for responding to nitrotoluenes.Microbial biotechnology · 2008Article
- The prokaryotic V4R domain is the likely ancestor of a key component of the eukaryotic vesicle transport system.Biology direct · 2008Review
- Mutant HbpR transcription activator isolation for 2-chlorobiphenyl via green fluorescent protein-based flow cytometry and cell sorting.Microbial biotechnology · 2008Article
- Selection of biocatalysts for chemical synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Laboratory-directed protein evolution.Microbiology and molecular biology reviews : MMBR · 2005Review
- Bacterial transcriptional regulators for degradation pathways of aromatic compounds.Microbiology and molecular biology reviews : MMBR · 2004Review
- Integration of global regulation of two aromatic-responsive sigma(54)-dependent systems: a common phenotype by different mechanisms.Journal of bacteriology · 2002Article
- New insights into the activation of o-xylene biodegradation in Pseudomonas stutzeri OX1 by pathway substrates.EMBO reports · 2001Article
- An active role for a structured B-linker in effector control of the sigma54-dependent regulator DmpR.The EMBO journal · 2001Article
- Role of the DmpR-mediated regulatory circuit in bacterial biodegradation properties in methylphenol-amended soils.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pseudomonas derived sigma(54)-dependent regulators DmpR and XylR control the expression of genes involved in catabolism of aromatic compounds. Binding to distinct, nonoverlapping groups of aromatic effectors controls the activities of these transcriptional activators. Previous work has derived a common mechanistic model for these two regulators in which effector binding by the N-terminal 210 residues (the A-domain) of the protein relieves repression of an intrinsic ATPase activity essential for its transcription-promoting property and allows productive interaction with the transcriptional apparatus. Here we dissect the A-domains of DmpR and XylR by DNA shuffling to identify the region(s) that mediates the differences in the effector specificity profiles. Analysis of in vivo transcription in response to multiple aromatic effectors and the in vitro phenol-binding abilities of regulator derivatives with hybrid DmpR/XylR A-domains reveals that residues 110 to 186 are key determinants that distinguish the effector profiles of DmpR and XylR. Moreover, the properties of some mosaic DmpR/XylR derivatives reveal that high-affinity aromatic effector binding can be completely uncoupled from the ability to promote transcription. Hence, novel aromatic binding properties will only be translated into functional transcriptional activation if effector binding also triggers release of interdomain repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.