ArticleBritish journal of pharmacology2000
Differential effects of UTP and ATP on ion transport in porcine tracheal epithelium.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Effect of Propolis Preparations on Transepithelial Electrical Potential, Resistance, and Ion Transport inEvidence-based complementary and alternative medicine : eCAM · 2019Article
- ENaCs and ASICs as therapeutic targets.American journal of physiology. Cell physiology · 2012Review
- Regulation of the epithelial Na+ channel and airway surface liquid volume by serine proteases.Pflugers Archiv : European journal of physiology · 2010Review
- Lipopolysaccharide modifies amiloride-sensitive Na+ transport processes across human airway cells: role of mitogen-activated protein kinases ERK 1/2 and 5.Pflugers Archiv : European journal of physiology · 2010Article
- ENaC, renal sodium excretion and extracellular ATP.Purinergic signalling · 2009Article
- Characteristics of Cl- uptake in rat alveolar type I cells.American journal of physiology. Lung cellular and molecular physiology · 2009Article
- Cation currents in human airway epithelial cells induced by infection with influenza A virus.The Journal of physiology · 2009Article
- Epithelial sodium channels in the adult lung--important modulators of pulmonary health and disease.Advances in experimental medicine and biology · 2007Review
- Adenosine-evoked Na+ transport in human airway epithelial cells.British journal of pharmacology · 2006Article
- Ion transport regulated by protease-activated receptor 2 in human airway Calu-3 epithelia.British journal of pharmacology · 2005Article
- Regulation of ion transport via apical purinergic receptors in intact rabbit airway epithelium.Pflugers Archiv : European journal of physiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated segments of porcine tracheal epithelium were mounted in Ussing chambers, current required to maintain transepithelial potential difference at 0 mV (short circuit current, I(SC)) was monitored and effects of nucleotides upon I(SC) were studied. Mucosal UTP (100 microM) evoked a transient rise in I(SC) that was followed by a sustained fall below basal I(SC) maintained for 30 min. Mucosal ATP (100 microM) also stimulated a transient rise in I(SC) but in contrast to UTP did not inhibit basal I(SC). Submucosal UTP and ATP both transiently increased I(SC). UTP-prestimulated epithelia were refractory to ATP but prestimulation with ATP did not abolish the response to UTP. The epithelia thus appear to express two populations of apical receptors allowing nucleotides to modulate I(SC). The UTP-induced rise was reduced by pretreatment with either bumetanide (100 microM), diphenylamin-2-carboxylic acid (DPC, 1 mM), or Cl(-) and HCO(3)(-)-free solution whilst the fall was abolished by amiloride pretreatment. Thapsigargin (0.3 microM) abolished the UTP-induced increase in I(SC) but not the subsequent decrease. Staurosporine (0.1 microM) inhibited basal I(SC) and blocked UTP-induced inhibition of I(SC). Inhibitors of either protein kinase C (PKC) (D-erythro sphingosine) or PKA (H89) had no effect. This study suggests that UTP stimulates Cl(-) secretion and inhibits basal Na(+) absorption. ATP has a similar stimulatory effect, which may be mediated by activation of P2Y(2) receptors and an increase in [Ca(2+)](in), but no inhibitory effect, which is likely mediated by activation of a pyrimidine receptor and possible inhibition of a protein kinase other than PKC or PKA.
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