ArticlePlant physiology2000
Regulation of high-affinity nitrate transporter genes and high-affinity nitrate influx by nitrogen pools in roots of barley.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 234 citations in OpenAlex.
- Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition.ISME communications · 2025Article
- Regulatory analysis of root architectural and anatomical adaptation to nitrate and ammonium inFrontiers in plant science · 2025Article
- Dissection of the spatial dynamics of biosynthesis, transport, and turnover of major amino acids in tea plants (Horticulture research · 2024Article
- Calcium regulates primary nitrate response associated gene transcription in a time- and dose-dependent manner.Protoplasma · 2024Article
- Biostimulant Properties of Protein Hydrolysates: Recent Advances and Future Challenges.International journal of molecular sciences · 2023Review
- Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo.Scientific reports · 2023Article
- Root nitrate uptake in sugarcane (Saccharum spp.) is modulated by transcriptional and presumably posttranscriptional regulation of the NRT2.1/NRT3.1 transport system.Molecular genetics and genomics : MGG · 2022Article
- Salt stress resilience in plants mediated through osmolyte accumulation and its crosstalk mechanism with phytohormones.Frontiers in plant science · 2022Review
- Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Plants (Basel, Switzerland) · 2020Article
- The Role of Gibberellins in Regulation of Nitrogen Uptake and Physiological Traits in Maize Responding to Nitrogen Availability.International journal of molecular sciences · 2020Article
- Glutamine application promotes nitrogen and biomass accumulation in the shoot of seedlings of the maize hybrid ZD958.Planta · 2020Article
- Transcriptome analysis of potato shoots, roots and stolons under nitrogen stress.Scientific reports · 2020Article
- Targeting Nitrogen Metabolism and Transport Processes to Improve Plant Nitrogen Use Efficiency.Frontiers in plant science · 2020Review
- Chromatin Remodeling Protein ZmCHB101 Regulates Nitrate-Responsive Gene Expression in Maize.Frontiers in plant science · 2020Article
- Genetic analyses of nitrogen assimilation enzymes in Brassica juncea (L.) Czern & Coss.Molecular biology reports · 2019Article
- Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability.Scientific reports · 2019Article
- Inhibition of Aminotransferases by Aminoethoxyvinylglycine Triggers a Nitrogen Limitation Condition and Deregulation of Histidine Homeostasis That Impact Root and Shoot Development and Nitrate Uptake.Frontiers in plant science · 2019Article
- Seed and Foliar Application of Amino Acids Improve Variables of Nitrogen Metabolism and Productivity in Soybean Crop.Frontiers in plant science · 2018Article
- Exogenous glycine inhibits root elongation and reduces nitrate-N uptake in pak choi (Brassica campestris ssp. Chinensis L.).PloS one · 2018Article
- Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom.The Plant cell · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the regulation of HvNRT2, genes that encode high-affinity NO(3)(-) transporters in barley (Hordeum vulgare) roots, seedlings were treated with 10 mM NO(3)(-) in the presence or absence of amino acids (aspartate, asparagine, glutamate [Glu], and glutamine [Gln]), NH(4)(+), and/or inhibitors of N assimilation. Although all amino acids decreased high-affinity (13)NO(3)(-) influx and HvNRT2 transcript abundance, there was substantial interconversion of administered amino acids, making it impossible to determine which amino acid(s) were responsible for the observed effects. To clarify the role of individual amino acids, plants were separately treated with tungstate, methionine sulfoximine, or azaserine (inhibitors of nitrate reductase, Gln synthetase, and Glu synthase, respectively). Tungstate increased the HvNRT2 transcript by 20% to 30% and decreased NO(3)(-) influx by 50%, indicating that NO(3)(-) itself does not regulate transcript abundance, but may exert post-transcriptional effects. Experiments with methionine sulfoximine suggested that NH(4)(+) may down-regulate HvNRT2 gene expression and high-affinity NO(3)(-) influx by effects operating at the transcriptional and post-transcriptional levels. Azaserine decreased HvNRT2 transcript levels and NO(3)(-) influx by 97% and 95%, respectively, while decreasing Glu and increasing Gln levels. This suggests that Gln (and not Glu) is responsible for down-regulating HvNRT2 expression, although it does not preclude a contributory effect of other amino acids.
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