ArticlePlant physiology2000
Sieve tube unloading and post-phloem transport of fluorescent tracers and proteins injected into sieve tubes via severed aphid stylets.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 75 citations in OpenAlex.
- Precision Delivery of Multiscale Payloads to Tissue-Specific Targets in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Plant-Aphid Interactions Under Elevated CO2: Some Cues from Aphid Feeding Behavior.Frontiers in plant science · 2016Review
- Article
- Metabolic Architecture of the Cereal Grain and Its Relevance to Maximize Carbon Use Efficiency.Plant physiology · 2015Article
- Observation and investigation of three endosperm transport tissues in sorghum caryopses.Protoplasma · 2015Article
- Plasmodesmata without callose and calreticulin in higher plants - open channels for fast symplastic transport?Frontiers in plant science · 2014Article
- Physical, metabolic and developmental functions of the seed coat.Frontiers in plant science · 2014Review
- Importance of symplasmic communication in cell differentiation.Plant signaling & behavior · 2014Review
- Role of metabolite transporters in source-sink carbon allocation.Frontiers in plant science · 2013Article
- Does Don Fisher's high-pressure manifold model account for phloem transport and resource partitioning?Frontiers in plant science · 2013Article
- Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.Journal of experimental botany · 2012Article
- The puzzle of phloem pressure.Plant physiology · 2010Article
- The role of phloem loading reconsidered.Plant physiology · 2010Article
- Changes in macromolecular movement accompany organogenesis in thin cell layers of Torenia fournieri.Planta · 2005Article
- Cell-to-cell movement of green fluorescent protein reveals post-phloem transport in the outer integument and identifies symplastic domains in Arabidopsis seeds and embryos.Plant physiology · 2005Article
- Soluble invertase expression is an early target of drought stress during the critical, abortion-sensitive phase of young ovary development in maize.Plant physiology · 2002Article
- L-Ascorbic acid is accumulated in source leaf phloem and transported to sink tissues in plants.Plant physiology · 2002Article
- Plasmodesma-mediated selective protein traffic between "symplasmically isolated" cells probed by a viral movement protein.The Plant cell · 2002Article
- Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading.The Plant cell · 2001Article
- Evidence for symplastic phloem unloading in sink leaves of barley.Plant physiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A variety of fluorescent tracers and proteins were injected via severed aphid stylets into the sieve tubes of wheat (Triticum aestivum L.) grains to evaluate the dimensions of plasmodesmal channels involved in sieve element/companion cell (SE/CC) unloading and post-phloem transport. In the post-phloem pathway, where diffusion is the predominant mode of transport, the largest molecule to show mobility was 16-kD dextran, with a Stokes radius of 2.6 nm. This suggests that the aqueous channels for cell-to-cell transport must be about 8 nm in diameter. Even the largest tracer injected into the sieve tubes, 400-kD fluorescein-labeled Ficoll with a Stokes radius of about 11 nm, was unloaded from the SE/CC complex. However, in contrast to smaller tracers (< or =3 kD, with a Stokes radius < or = 1.2 nm), the unloading of fluorescein-labeled Ficoll and other large molecules from the SE/CC complex showed an irregular, patchy distribution, with no further movement along the post-phloem pathway. Either the plasmodesmal channels involved in SE/CC unloading are exceptionally large (perhaps as much as 42 nm in diameter), with only a very small fraction of plasmodesmata being conductive, or the larger tracers damage the plasmodesmata in some way, enlarging smaller channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.