ArticleProceedings of the National Academy of Sciences of the United States of America2000
LexA chimeras reveal the function of Drosophila Fos as a context-dependent transcriptional activator.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
- EyaHOST, a modular genetic system for investigation of intercellular and tumor-host interactions in Drosophila melanogaster.Cell reports methods · 2025Article
- Selective optogenetic inhibition of GαProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.G3 (Bethesda, Md.) · 2023Article
- REPTOR and CREBRF encode key regulators of muscle energy metabolism.Nature communications · 2023Article
- Spatial and temporal control of expression with light-gated LOV-LexA.G3 (Bethesda, Md.) · 2022Article
- Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ.Journal of cell science · 2022Article
- Molecular and cellular basis of acid taste sensation in Drosophila.Nature communications · 2021Article
- The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction.Nature communications · 2021Article
- Identification ofOpen biology · 2020Article
- RNA-guided retargeting of SeLife · 2020Article
- The fight to understand fighting: neurogenetic approaches to the study of aggression in insects.Current opinion in insect science · 2019Review
- The Drosophila ERG channel seizure plays a role in the neuronal homeostatic stress response.PLoS genetics · 2019Article
- An Interscholastic Network To Generate LexA Enhancer Trap Lines inG3 (Bethesda, Md.) · 2019Article
- TheJournal of cell science · 2018Article
- Angiotensin-converting enzyme Ance is cooperatively regulated by Mad and Pannier in Drosophila imaginal discs.Scientific reports · 2017Article
- The equilibrium between antagonistic signaling pathways determines the number of synapses in Drosophila.PloS one · 2017Article
- A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research.G3 (Bethesda, Md.) · 2016Article
- CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila.Development (Cambridge, England) · 2015Article
- TIE-DYE: a combinatorial marking system to visualize and genetically manipulate clones during development in Drosophila melanogaster.Development (Cambridge, England) · 2013Article
- Retargeting sleeping beauty transposon insertions by engineered zinc finger DNA-binding domains.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional activation potential of proteins can be assayed in chimeras containing a heterologous DNA-binding domain that mediates their recruitment to reporter genes. This approach has been widely used in yeast and in transient mammalian cell assays. Here, we applied it to assay the transactivation potential of proteins in transgenic Drosophila embryos. We found that a chimera between the DNA-binding bacterial LexA protein and the transactivation domain from yeast GAL4 behaved as a potent synthetic activator in all embryonic tissues. In contrast, a LexA chimera containing Drosophila Fos (Dfos) required an unexpected degree of context to function as a transcriptional activator. We provide evidence to suggest that this context is provided by Djun and Mad (a Drosophila Smad), and that these partner factors need to be activated by signaling from Jun N-terminal kinase and decapentaplegic, respectively. Because Dfos behaves as an autonomous transcriptional activator in more artificial assays systems, our data suggest that context-dependence of transcription factors may be more prevalent than previously thought.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.