ArticleProceedings of the National Academy of Sciences of the United States of America2000
Role of Rac in controlling the actin cytoskeleton and chemotaxis in motile cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 148 citations in OpenAlex.
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- PTEN and the PTEN-like phosphatase CnrN have both distinct and overlapping roles in a Dictyostelium chemorepulsion pathway.Journal of cell science · 2024Article
- Endolysosomal TRPML1 channel regulates cancer cell migration by altering intracellular trafficking of E-cadherin and βThe Journal of biological chemistry · 2024Article
- The CXCR4 antagonist R54 targets epithelial-mesenchymal transition (EMT) in human ovarian cancer cells.PloS one · 2024Article
- Phosphoinositide Signaling in Immune Cell Migration.Biomolecules · 2023Review
- The therapeutic potential of neurofibromin signaling pathways and binding partners.Communications biology · 2023Review
- Coronin 2B Regulates Neuronal Migration via Rac1-Dependent Multipolar-Bipolar Transition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- MicroRNAs as the critical regulators of cell migration and invasion in thyroid cancer.Biomarker research · 2022Review
- Rho Kinase regulates neutrophil NET formation that is involved in UVB-induced skin inflammation.Theranostics · 2022Article
- Myosin II and Arp2/3 cross-talk governs intracellular hydraulic pressure and lamellipodia formation.Molecular biology of the cell · 2021Article
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
- Coordination between Rac1 and Rab Proteins: Functional Implications in Health and Disease.Cells · 2019Review
- Rac activation is key to cell motility and directionality: An experimental and modelling investigation.Computational and structural biotechnology journal · 2019Article
- Inhibition of microRNA suppression ofDevelopment (Cambridge, England) · 2018Article
- Involvement of Rho GAP GRAF1 in maintenance of epithelial phenotype.Cell adhesion & migration · 2017Article
- WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.The Journal of cell biology · 2017Article
- Biophysical Tools to Study Cellular Mechanotransduction.Bioengineering (Basel, Switzerland) · 2017Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used the chemotactic ability of Dictyostelium cells to examine the roles of Rho family members, known regulators of the assembly of F-actin, in cell movement. Wild-type cells polarize with a leading edge enriched in F-actin toward a chemoattractant. Overexpression of constitutively active Dictyostelium Rac1B(61L) or disruption of DdRacGAP1, which encodes a Dictyostelium Rac1 GAP, induces membrane ruffles enriched with actin filaments around the perimeter of the cell and increased levels of F-actin in resting cells. Whereas wild-type cells move linearly toward the cAMP source, Rac1B(61L) and Ddracgap1 null cells make many wrong turns and chemotaxis is inefficient, which presumably results from the unregulated activation of F-actin assembly and pseudopod extension. Cells expressing dominant-negative DdRac1B(17N) do not have a well-defined F-actin-rich leading edge and do not protrude pseudopodia, resulting in very poor cell motility. From these studies and assays examining chemoattractant-mediated F-actin assembly, we suggest DdRac1 regulates the basal levels of F-actin assembly, its dynamic reorganization in response to chemoattractants, and cellular polarity during chemotaxis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.