ArticleMolecular and cellular biology2000
S-Phase progression mediates activation of a silenced gene in synthetic nuclei.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Cell-cycle dependence of transcription dominates noise in gene expression.PLoS computational biology · 2013Article
- p53-targeted LSD1 functions in repression of chromatin structure and transcription in vivo.Molecular and cellular biology · 2008Article
- Zinc finger protein ZBTB20 is a key repressor of alpha-fetoprotein gene transcription in liver.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts.Nucleic acids research · 2006Article
- A direct intersection between p53 and transforming growth factor beta pathways targets chromatin modification and transcription repression of the alpha-fetoprotein gene.Molecular and cellular biology · 2005Article
- Vertebrate HoxB gene expression requires DNA replication.The EMBO journal · 2003Article
- Chromatin alteration, transcription and replication: What's the opening line to the story?Oncogene · 2001Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Aberrant expression of developmentally silenced genes, characteristic of tumor cells and regenerating tissue, is highly correlated with increased cell proliferation. By modeling this process in vitro in synthetic nuclei, we find that DNA replication leads to deregulation of established developmental expression patterns. Chromatin assembly in the presence of adult mouse liver nuclear extract mediates developmental stage-specific silencing of the tumor marker gene alpha-fetoprotein (AFP). Replication of silenced AFP chromatin in synthetic nuclei depletes sequence-specific transcription repressors, thereby disrupting developmentally regulated repression. Hepatoma-derived factors can target partial derepression of AFP, but full transcription activation requires DNA replication. Thus, unscheduled entry into S phase directly mediates activation of a developmentally silenced gene by (i) depleting developmental stage-specific transcription repressors and (ii) facilitating binding of transactivators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.