ArticleMolecular and cellular biology2000
A novel chromodomain protein, pdd3p, associates with internal eliminated sequences during macronuclear development in Tetrahymena thermophila.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Review
- A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies.Nucleic acids research · 2021Article
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- A mating-type mutagenesis screen identifies a zinc-finger protein required for specific DNA excision events in Paramecium.Nucleic acids research · 2018Article
- Diversification of HP1-like Chromo Domain Proteins in Tetrahymena thermophila.The Journal of eukaryotic microbiology · 2018Article
- The Co-regulation Data Harvester: automating gene annotation starting from a transcriptome database.SoftwareX · 2017Article
- The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila.Genes & development · 2016Article
- A Parallel G Quadruplex-Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila.PLoS genetics · 2016Article
- Chromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in Tetrahymena.Scientific reports · 2015Article
- Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena.Journal of cell science · 2015Article
- Tetrahymena Pot2 is a developmentally regulated paralog of Pot1 that localizes to chromosome breakage sites but not to telomeres.Eukaryotic cell · 2014Article
- Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination.Nucleic acids research · 2014Article
- Beyond transcriptional silencing: is methylcytosine a widely conserved eukaryotic DNA elimination mechanism?BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Article
- Mutations in Pdd1 reveal distinct requirements for its chromodomain and chromoshadow domain in directing histone methylation and heterochromatin elimination.Eukaryotic cell · 2014Article
- Review
- Article
- Cytosine methylation and hydroxymethylation mark DNA for elimination in Oxytricha trifallax.Genome biology · 2012Article
- Tetrahymena thermophila JMJD3 homolog regulates H3K27 methylation and nuclear differentiation.Eukaryotic cell · 2012Article
- Zygotic expression of the double-stranded RNA binding motif protein Drb2p is required for DNA elimination in the ciliate Tetrahymena thermophila.Eukaryotic cell · 2011Article
- Keeping the soma free of transposons: programmed DNA elimination in ciliates.The Journal of biological chemistry · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conversion of the germ line micronuclear genome into the genome of a somatic macronucleus in Tetrahymena thermophila requires several DNA rearrangement processes. These include (i) excision and subsequent elimination of several thousand internal eliminated sequences (IESs) scattered throughout the micronuclear genome and (ii) breakage of the micronuclear chromosomes into hundreds of DNA fragments, followed by de novo telomere addition to their ends. Chromosome breakage sequences (Cbs) that determine the sites of breakage and short regions of DNA adjacent to them are also eliminated. Both processes occur concomitantly in the developing macronucleus. Two stage-specific protein factors involved in germ line DNA elimination have been described previously. Pdd1p and Pdd2p (for programmed DNA degradation) physically associate with internal eliminated sequences in transient electron-dense structures in the developing macronucleus. Here, we report the purification, sequence analysis, and characterization of Pdd3p, a novel developmentally regulated, chromodomain-containing polypeptide. Pdd3p colocalizes with Pdd1p in the peripheral regions of DNA elimination structures, but is also found more internally. DNA cross-linked and immunoprecipitated with Pdd1p- or Pdd3p-specific antibodies is enriched in IESs, but not Cbs, suggesting that different protein factors are involved in elimination of these two groups of sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.