ArticleMolecular and cellular biology2000
hnRNP C is required for postimplantation mouse development but Is dispensable for cell viability.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- HNRNPC haploinsufficiency affects alternative splicing of intellectual disability-associated genes and causes a neurodevelopmental disorder.American journal of human genetics · 2023Pooled it
- Multiomics profiling uncovers curdione-induced reproductive toxicity in HTR-8/SVneo cells.Heliyon · 2024Article
- Article
- FOXM1a Isoform of Oncogene FOXM1 Is a Tumor Suppressor Suppressed by hnRNP C in Oral Squamous Cell Carcinoma.Biomolecules · 2023Article
- An analysis of the role of HnRNP C dysregulation in cancers.Biomarker research · 2022Review
- Nhlrc2 is crucial during mouse gastrulation.Genesis (New York, N.Y. : 2000) · 2022Article
- Alternative RNA splicing in tumour heterogeneity, plasticity and therapy.Disease models & mechanisms · 2022Review
- RRM but not the Asp/Glu domain of hnRNP C1/C2 is required for splicing regulation of Ron exon 11 pre-mRNA.BMB reports · 2019Article
- Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response.The EMBO journal · 2018Article
- Proteomic analysis of polyribosomes identifies splicing factors as potential regulators of translation during mitosis.Nucleic acids research · 2017Article
- The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.Molecular and cellular biology · 2016Article
- Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination.PloS one · 2013Article
- PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis.Molecular and cellular biology · 2009Article
- DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice.RNA (New York, N.Y.) · 2008Article
- Regulation of protein kinase CK1alphaLS by dephosphorylation in response to hydrogen peroxide.Archives of biochemistry and biophysics · 2007Article
- The impact of alternative splicing in vivo: mouse models show the way.RNA (New York, N.Y.) · 2007Review
- Downregulation of hnRNP C1/C2 by siRNA sensitizes HeLa cells to various stresses.Molecular and cellular biochemistry · 2007Article
- Hypomorphic mutation in hnRNP U results in post-implantation lethality.Transgenic research · 2005Article
- Activation of cryptic 3' splice sites within introns of cellular genes following gene entrapment.Nucleic acids research · 2004Article
- The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The hnRNP C1 and C2 proteins are among the most abundant proteins in the nucleus, and as ubiquitous components of RNP complexes, they have been implicated in many aspects of mRNA biogenesis. In this report, we have characterized a null mutation induced in embryonic stem cells by insertion of the U3His gene trap retrovirus into the first intron of the hnRNP C1/C2 gene. cDNAs encoding murine hnRNP C1 and C2 were characterized, and the predicted protein sequences were found to be highly conserved among vertebrates. A human consensus sequence, generated from over 400 expressed sequence tags, suggests two revisions to the previously published human sequence. In addition, alternatively spliced transcripts, expressed only by the murine gene, encode four novel proteins: variants of C1 and C2 with either seven additional amino acids or one fewer amino acid in a region between the oligomerization and C-terminal acidic domains. The disrupted gene was transmitted into the germ line and is tightly linked to a recessive, embryonic lethal phenotype. Homozygous mutant embryos fail to develop beyond the egg cylinder stage and are resorbed by 10.5 days of gestation, a phenotype consistent with a fundamental role in cellular metabolism. However, hnRNP C1 and C2 are not required for cell viability. Embryonic stem cell lines established from homozygous mutant blastocysts did not express detectable levels of either protein yet were able to grow and differentiate in vitro, albeit more slowly than wild-type cells. These results indicate that the C1 and C2 hnRNPs are not required for any essential step in mRNA biogenesis; however, the proteins may influence the rate and/or fidelity of one or more steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.