ArticleMolecular and cellular biology2000
Homologous and nonhomologous recombination resulting in deletion: effects of p53 status, microhomology, and repetitive DNA length and orientation.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 98 citations in OpenAlex.
- A founder large deletion mutation in Xeroderma pigmentosum-Variant form in Tunisia: implication for molecular diagnosis and therapy.BioMed research international · 2014Trial
- Mutagenic assessment of chemotherapy and Smac mimetic drugs in cells with defective DNA damage response pathways.Scientific reports · 2018Article
- DNA Damage-Response Pathway Heterogeneity of Human Lung Cancer A549 and H1299 Cells Determines Sensitivity to 8-Chloro-Adenosine.International journal of molecular sciences · 2018Article
- Divergent genome evolution caused by regional variation in DNA gain and loss between human and mouse.PLoS computational biology · 2018Article
- The contribution of alu elements to mutagenic DNA double-strand break repair.PLoS genetics · 2015Article
- Association of new deletion/duplication region at chromosome 1p21 with intellectual disability, severe speech deficit and autism spectrum disorder-like behavior: an all-in approach to solving theTranslational neuroscience · 2015Review
- One in four individuals of African-American ancestry harbors a 5.5kb deletion at chromosome 11q13.1.Genomics · 2014Article
- DNA repair pathway gene expression score correlates with repair proficiency and tumor sensitivity to chemotherapy.Science translational medicine · 2014Article
- Comparative analysis of the inverted repeat of a chalcone synthase pseudogene between yellow soybean and seed coat pigmented mutants.Breeding science · 2013Article
- Alu elements: an intrinsic source of human genome instability.Current opinion in virology · 2013Review
- Comprehensive analysis of the genome transcriptome and proteome landscapes of three tumor cell lines.Genome medicine · 2012Article
- Modes of retrotransposition of long interspersed element-1 by environmental factors.Frontiers in microbiology · 2012Article
- All y'all need to know 'bout retroelements in cancer.Seminars in cancer biology · 2010Review
- Genomic characterization of large rearrangements of the LDLR gene in Czech patients with familial hypercholesterolemia.BMC medical genetics · 2010Article
- Mapping the physical and functional interactions between the tumor suppressors p53 and BRCA2.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- L1 recombination-associated deletions generate human genomic variation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Chromosomal inversions between human and chimpanzee lineages caused by retrotransposons.PloS one · 2008Article
- Low copy repeats mediate distal chromosome 22q11.2 deletions: sequence analysis predicts breakpoint mechanisms.Genome research · 2007Article
- Inviting instability: Transposable elements, double-strand breaks, and the maintenance of genome integrity.Mutation research · 2007Review
- UV radiation induces delayed hyperrecombination associated with hypermutation in human cells.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Repetitive DNA elements frequently are precursors to chromosomal deletions in prokaryotes and lower eukaryotes. However, little is known about the relationship between repeated sequences and deletion formation in mammalian cells. We have created a novel integrated plasmid-based recombination assay to investigate repeated sequence instability in human cells. In a control cell line, the presence of direct or inverted repeats did not appreciably influence the very low deletion frequencies (2 x 10(-7) to 9 x 10(-7)) in the region containing the repeat. Similar to what has been observed in lower eukaryotes, the majority of deletions resulted from the loss of the largest direct repeat present in the system along with the intervening sequence. Interestingly, in closely related cell lines that possess a mutant p53 gene, deletion frequencies in the control and direct-repeat plasmids were 40 to 300 times higher than in their wild-type counterparts. However, mutant p53 cells did not preferentially utilize the largest available homology in the formation of the deletion. Surprisingly, inverted repeats were approximately 10,000 times more unstable in all mutant p53 cells than in wild-type cells. Finally, several deletion junctions were marked by the addition of novel bases that were homologous to one of the preexisting DNA ends. Contrary to our expectations, only 6% of deletions in all cell lines could be classified as arising from nonhomologous recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.