ArticleMolecular and cellular biology2000
A 5' splice site-proximal enhancer binds SF1 and activates exon bridging of a microexon.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Phylogenomic profile of exon-intron organization across angiosperms, their relationships with protein domains, and functional implications.Nature communications · 2026Article
- RNA-binding proteins connect Exon usage to the chromatin.NAR genomics and bioinformatics · 2025Article
- Exon definitive regions forMolecular therapy. Nucleic acids · 2023Article
- Selection of Olduvai Domains during Evolution: A Role for Primate-Specific Splicing Super-Enhancer and RNA Guanine Quadruplex in BipartiteBrain sciences · 2022Article
- Pervasive misannotation of microexons that are evolutionarily conserved and crucial for gene function in plants.Nature communications · 2022Article
- Microexons: discovery, regulation, and function.Wiley interdisciplinary reviews. RNA · 2017Review
- RBFOX and PTBP1 proteins regulate the alternative splicing of micro-exons in human brain transcripts.Genome research · 2015Article
- OLego: fast and sensitive mapping of spliced mRNA-Seq reads using small seeds.Nucleic acids research · 2013Article
- Article
- G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.The Journal of biological chemistry · 2011Article
- Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs.The Journal of biological chemistry · 2009Article
- Dual-specificity splice sites function alternatively as 5' and 3' splice sites.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.PLoS biology · 2006Article
- Synonymous SNPs provide evidence for selective constraint on human exonic splicing enhancers.Journal of molecular evolution · 2006Article
- Sequence conservation, relative isoform frequencies, and nonsense-mediated decay in evolutionarily conserved alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A combinatorial code for splicing silencing: UAGG and GGGG motifs.PLoS biology · 2005Article
- Sequence information for the splicing of human pre-mRNA identified by support vector machine classification.Genome research · 2003Article
- Perturbation of transcription elongation influences the fidelity of internal exon inclusion in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2003Article
- Conserved sequence elements associated with exon skipping.Nucleic acids research · 2003Article
- Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Internal exon size in vertebrates occurs over a narrow size range. Experimentally, exons shorter than 50 nucleotides are poorly included in mRNA unless accompanied by strengthened splice sites or accessory sequences that act as splicing enhancers, suggesting steric interference between snRNPs and other splicing factors binding simultaneously to the 3' and 5' splice sites of microexons. Despite these problems, very small naturally occurring exons exist. Here we studied the factors and mechanism involved in recognizing a constitutively included six-nucleotide exon from the cardiac troponin T gene. Inclusion of this exon is dependent on an enhancer located downstream of the 5' splice site. This enhancer contains six copies of the simple sequence GGGGCUG. The enhancer activates heterologous microexons and will work when located either upstream or downstream of the target exon, suggesting an ability to bind factors that bridge splicing units. A single copy of this sequence is sufficient for in vivo exon inclusion and is the binding site for the known bridging mammalian splicing factor 1 (SF1). The enhancer and its bound SF1 act to increase recognition of the upstream exon during exon definition, such that competition of in vitro reactions with RNAs containing the GGGGCUG repeated sequence depress splicing of the upstream intron, assembly of the spliceosome on the 3' splice site of the exon, and cross-linking of SF1. These results suggest a model in which SF1 bridges the small exon during initial assembly, thereby effectively extending the domain of the exon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.