ArticleJournal of virology2000
Topoisomerase I associates specifically with simian virus 40 large-T-antigen double hexamer-origin complexes.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- DNA damage-induced phosphorylation of a replicative DNA helicase results in inhibition of DNA replication through attenuation of helicase function.Nucleic acids research · 2024Article
- The HPV E2 Transcriptional Transactivation Protein Stimulates Cellular DNA Polymerase Epsilon.Viruses · 2018Article
- The E1 proteins.Virology · 2013Review
- Structural and functional interactions of the prostate cancer suppressor protein NKX3.1 with topoisomerase I.The Biochemical journal · 2013Article
- Simian virus 40 DNA replication is dependent on an interaction between topoisomerase I and the C-terminal end of T antigen.Journal of virology · 2008Article
- Role of the hydrophilic channels of simian virus 40 T-antigen helicase in DNA replication.Journal of virology · 2007Article
- Cellular topoisomerase I modulates origin binding by bovine papillomavirus type 1 E1.Journal of virology · 2006Article
- Assembly of the replication initiation complex on SV40 origin DNA.Nucleic acids research · 2004Article
- Nonspecific double-stranded DNA binding activity of simian virus 40 large T antigen is involved in melting and unwinding of the origin.Journal of virology · 2003Article
- The cap region of topoisomerase I binds to sites near both ends of simian virus 40 T antigen.Journal of virology · 2003Article
- Bidirectional DNA unwinding by a ternary complex of T antigen, nucleolin and topoisomerase I.EMBO reports · 2003Article
- Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein.Molecular and cellular biology · 2002Article
- Induction of the bovine papillomavirus origin "onion skin"-type DNA replication at high E1 protein concentrations in vivo.Journal of virology · 2002Article
- Mutational analysis of simian virus 40 T-antigen primosome activities in viral DNA replication.Journal of virology · 2002Article
- In the simian virus 40 in vitro replication system, start site selection by the polymerase alpha-primase complex is not significantly altered by changes in the concentration of ribonucleotides.Journal of virology · 2001Article
- Role of single-stranded DNA binding activity of T antigen in simian virus 40 DNA replication.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Topoisomerase I (topo I) is required for releasing torsional stress during simian virus 40 (SV40) DNA replication. Recently, it has been demonstrated that topo I participates in initiation of replication as well as in elongation. Although T antigen and topo I can bind to one another in vitro, there is no direct evidence that topo I is a component of the replication initiation complex. We demonstrate in this report that topo I associates with T-antigen double hexamers bound to SV40 origin DNA (T(DH)) but not to single hexamers. This association has the same nucleotide and DNA requirements as those for the formation of double hexamers on DNA. Interestingly, topo I prefers to bind to fully formed T(DH) complexes over other oligomerized forms of T antigen associated with the origin. High ratios of topo I to origin DNA destabilize T(DH). The partial unwinding of a small-circular-DNA substrate is dependent on the presence of both T antigen and topo I but is inhibited at high topo I concentrations. Competition experiments with a topo I-binding fragment of T antigen indicate that an interaction between T antigen and topo I occurs during the unwinding reaction. We propose that topo I is recruited to the initiation complex after the assembly of T(DH) and before unwinding to facilitate DNA replication.
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