ArticleJournal of virology2000
Mutational analysis of the rubella virus nonstructural polyprotein and its cleavage products in virus replication and RNA synthesis.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Exogenous Rubella Virus Capsid Proteins Enhance Virus Genome Replication.Pathogens (Basel, Switzerland) · 2022Article
- Molecular and Structural Insights into the Life Cycle of Rubella Virus.Journal of virology · 2021Review
- Heat Shock Protein 90 Ensures the Integrity of Rubella Virus p150 Protein and Supports Viral Replication.Journal of virology · 2019Article
- Hepatitis E Virus Genome Structure and Replication Strategy.Cold Spring Harbor perspectives in medicine · 2019Review
- Activities of Thrombin and Factor Xa Are Essential for Replication of Hepatitis E Virus and Are Possibly Implicated in ORF1 Polyprotein Processing.Journal of virology · 2018Article
- Short self-interacting N-terminal region of rubella virus capsid protein is essential for cooperative actions of capsid and nonstructural p150 proteins.Journal of virology · 2014Article
- Determinants in the maturation of rubella virus p200 replicase polyprotein precursor.Journal of virology · 2012Article
- Lack of processing of the expressed ORF1 gene product of hepatitis E virus.Virology journal · 2011Article
- Proteolytic processing of turnip yellow mosaic virus replication proteins and functional impact on infectivity.Journal of virology · 2007Article
- Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein.Virology journal · 2006Article
- The involvement of survival signaling pathways in rubella-virus induced apoptosis.Virology journal · 2005Article
- Rubella virus capsid protein modulates viral genome replication and virus infectivity.Journal of virology · 2004Article
- Analysis of the 3' cis-acting elements of rubella virus by using replicons expressing a puromycin resistance gene.Journal of virology · 2004Article
- Complementation of a deletion in the rubella virus p150 nonstructural protein by the viral capsid protein.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rubella virus nonstructural proteins, translated from input genomic RNA as a p200 polyprotein and subsequently processed into p150 and p90 by an intrinsic papain-like thiol protease, are responsible for virus replication. To examine the effect of p200 processing on virus replication and to study the roles of nonstructural proteins in viral RNA synthesis, we introduced into a rubella virus infectious cDNA clone a panel of mutations that had variable defective effects on p200 processing. The virus yield and viral RNA synthesis of these mutants were examined. Mutations that completely abolished (C1152S and G1301S) or largely abolished (G1301A) cleavage of p200 resulted in noninfectious virus. Mutations that partially impaired cleavage of p200 (R1299A and G1300A) decreased virus replication. An RNase protection assay revealed that all of the mutants synthesized negative-strand RNA as efficiently as the wild type does but produced lower levels of positive-strand RNA. Our results demonstrated that processing of rubella virus nonstructural protein is crucial for virus replication and that uncleaved p200 could function in negative-strand RNA synthesis, whereas the cleavage products p150 and p90 are required for efficient positive-strand RNA synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.