ArticlePlant molecular biology2000
Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.
Article in Plant molecular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- Effects of fluorescent tags and activity status on the membrane localization of ROP GTPases.Plant signaling & behavior · 2024Article
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond.Plant communications · 2023Review
- ROP GTPases Structure-Function and Signaling Pathways.Plant physiology · 2018Review
- The Microtubule-Associated Protein MAP18 Affects ROP2 GTPase Activity during Root Hair Growth.Plant physiology · 2017Article
- Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance toFrontiers in plant science · 2017Article
- Arabidopsis RhoGDIs Are Critical for Cellular Homeostasis of Pollen Tubes.Plant physiology · 2016Article
- The Ectopic Expression of CaRop1 Modulates the Response of Tobacco Plants to Ralstonia solanacearum and Aphids.Frontiers in plant science · 2016Article
- CPK3-phosphorylated RhoGDI1 is essential in the development of Arabidopsis seedlings and leaf epidermal cells.Journal of experimental botany · 2013Article
- Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121.The Plant cell · 2012Article
- The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus.Plant physiology · 2012Article
- RopGEF7 regulates PLETHORA-dependent maintenance of the root stem cell niche in Arabidopsis.The Plant cell · 2011Article
- RHO GTPase in plants: Conservation and invention of regulators and effectors.Small GTPases · 2010Article
- Pollen-tube tip growth requires a balance of lateral propagation and global inhibition of Rho-family GTPase activity.Journal of cell science · 2010Article
- RIP1 (ROP Interactive Partner 1)/ICR1 marks pollen germination sites and may act in the ROP1 pathway in the control of polarized pollen growth.Molecular plant · 2008Article
- The Arabidopsis small G protein ROP2 is activated by light in guard cells and inhibits light-induced stomatal opening.The Plant cell · 2008Article
- A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Article
- Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum.The Plant cell · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (AtROP4 and AtROP6), we have generated translational fusions with the green fluorescent protein (GFP). Microscopic analysis of transiently transfected BY2 cells revealed a predominant localization of AtROP4 in the perinuclear region, while AtROP6 was localized almost exclusively to the plasma membrane. Swapping of the AtROP4 and AtROP6 C-termini produced a change in localization. As RhoGDIs are known to bind to the C-terminus of GTPases of the Rho family, we searched for Arabidopsis RhoGDI genes. We identified the AtRhoGDI1 gene and mapped it to chromosome 3. AtRhoGDI1 encodes a 22.5 kDa protein which contains highly conserved amino acids in the isoprene binding pocket and exhibits 29% to 37% similarity to known mammalian RhoGDI homologues. The AtRhoGDI1 gene was expressed in all tissues studied. Using the yeast two-hybrid system, we showed specific interaction of AtRhoGDI1 with both AtROP4 and AtROP6 as well as with their GTP-locked mutants, but not with a GTPase of the RAB family. Recombinant GST-AtRhoGDI1 could bind GFP-AtROP4 from transgenic tobacco BY2 cell extracts, confirming the interaction observed with the two-hybrid system.
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