ArticleMolecular biology of the cell2000
A novel selection regime for differentiation defects demonstrates an essential role for the stumpy form in the life cycle of the African trypanosome.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- The Occurrence of Malignancy inFrontiers in microbiology · 2021Article
- NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway.Nucleic acids research · 2015Article
- Article
- Transmission stages dominate trypanosome within-host dynamics during chronic infections.Cell host & microbe · 2011Article
- Developmental regulation and extracellular release of a VSG expression-site-associated gene product from Trypanosoma brucei bloodstream forms.Journal of cell science · 2010Article
- Trypanosoma brucei: reduction of GPI-phospholipase C protein during differentiation is dependent on replication of newly transformed cells.Experimental parasitology · 2010Article
- A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.Genes & development · 2010Article
- Article
- Role of the Trypanosoma brucei natural cysteine peptidase inhibitor ICP in differentiation and virulence.Molecular microbiology · 2007Article
- Hydrolysis products of cAMP analogs cause transformation of Trypanosoma brucei from slender to stumpy-like forms.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Protein tyrosine phosphatase TbPTP1: A molecular switch controlling life cycle differentiation in trypanosomes.The Journal of cell biology · 2006Article
- Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein.Molecular microbiology · 2005Article
- Cold shock and regulation of surface protein trafficking convey sensitization to inducers of stage differentiation in Trypanosoma brucei.Genes & development · 2004Article
- Stage-specific requirement of a mitogen-activated protein kinase by Trypanosoma brucei.Molecular biology of the cell · 2002Article
- Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements.Nucleic acids research · 2002Article
- Mitochondrial development during life cycle differentiation of African trypanosomes: evidence for a kinetoplast-dependent differentiation control point.Molecular biology of the cell · 2002Article
- Genetic analysis of phenotype in Trypanosoma brucei: a classical approach to potentially complex traits.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Article
- A novel CCCH protein which modulates differentiation of Trypanosoma brucei to its procyclic form.The EMBO journal · 2001Article
- Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels.Molecular microbiology · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A novel selection scheme has been developed to isolate bloodstream forms of Trypanosoma brucei, which are defective in their ability to differentiate to the procyclic stage. Detailed characterization of one selected cell line (defective in differentiation clone 1 [DiD-1]) has demonstrated that these cells are indistinguishable from the wild-type population in terms of their morphology, cell cycle progression, and biochemical characteristics but are defective in their ability to initiate differentiation to the procyclic form. Although a small proportion of DiD-1 cells remain able to transform, deletion of the genes for glycophosphatidyl inositol-phospholipase C demonstrated that this enzyme was not responsible for this inefficient differentiation. However, the attenuated growth of the Delta-glycophosphatidyl inositol-phospholipase C DiD-1 cells in mice permitted the expression of stumpy characteristics in this previously monomorphic cell line, and concomitantly their ability to differentiate efficiently was restored. Our results indicate that monomorphic cells retain expression of a characteristic of the stumpy form essential for differentiation, and that this is reduced in the defective cells. This approach provides a new route to dissection of the cytological and molecular basis of life cycle progression in the African trypanosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.