ArticleMolecular biology of the cell2000
Structure-function relationships in yeast tubulins.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.The Journal of cell biology · 2025Article
- Arginylation of ⍺-tubulin at E77 regulates microtubule dynamics via MAP1S.The Journal of cell biology · 2025Article
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- A distinct P-body-like granule is induced in response to the disruption of microtubule integrity in Saccharomyces cerevisiae.Genetics · 2022Article
- Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species.Current genetics · 2022Article
- Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.The Journal of cell biology · 2021Article
- Epistasis, aneuploidy, and functional mutations underlie evolution of resistance to induced microtubule depolymerization.The EMBO journal · 2021Article
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- Using Budding Yeast to Identify Molecules That Block Cancer Cell 'Mitotic Slippage' Only in the Presence of Mitotic Poisons.International journal of molecular sciences · 2021Review
- Nile Tilapia Derived Antimicrobial Peptide TP4 Exerts Antineoplastic Activity Through Microtubule Disruption.Marine drugs · 2018Article
- Structural and functional differences between porcine brain and budding yeast microtubules.Cell cycle (Georgetown, Tex.) · 2018Article
- Distinct effects of tubulin isotype mutations on neurite growth inMolecular biology of the cell · 2017Article
- Structural differences between yeast and mammalian microtubules revealed by cryo-EM.The Journal of cell biology · 2017Article
- An electron microscopy journey in the study of microtubule structure and dynamics.Protein science : a publication of the Protein Society · 2015Review
- Article
- Genome-wide analysis reveals novel and discrete functions for tubulin carboxy-terminal tails.Current biology : CB · 2014Article
- Article
- ε-tubulin is essential in Tetrahymena thermophila for the assembly and stability of basal bodies.Journal of cell science · 2013Article
- Structure-function analysis of yeast tubulin.Methods in cell biology · 2013Article
- An inherited TUBB2B mutation alters a kinesin-binding site and causes polymicrogyria, CFEOM and axon dysinnervation.Human molecular genetics · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A comprehensive set of clustered charged-to-alanine mutations was generated that systematically alter TUB1, the major alpha-tubulin gene of Saccharomyces cerevisiae. A variety of phenotypes were observed, including supersensitivity and resistance to the microtubule-destabilizing drug benomyl, lethality, and cold- and temperature-sensitive lethality. Many of the most benomyl-sensitive tub1 alleles were synthetically lethal in combination with tub3Delta, supporting the idea that benomyl supersensitivity is a rough measure of microtubule instability and/or insufficiency in the amount of alpha-tubulin. The systematic tub1 mutations were placed, along with the comparable set of tub2 mutations previously described, onto a model of the yeast alpha-beta-tubulin dimer based on the three-dimensional structure of bovine tubulin. The modeling revealed a potential site for binding of benomyl in the core of beta-tubulin. Residues whose mutation causes cold sensitivity were concentrated at the lateral and longitudinal interfaces between adjacent subunits. Residues that affect binding of the microtubule-binding protein Bim1p form a large patch across the exterior-facing surface of alpha-tubulin in the model. Finally, the positions of the mutations suggest that proximity to the alpha-beta interface may account for the finding of synthetic lethality of five viable tub1 alleles with the benomyl-resistant but otherwise entirely viable tub2-201 allele.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.