ArticleMolecular biology of the cell2000
Intercellular calcium waves in HeLa cells expressing GFP-labeled connexin 43, 32, or 26.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 133 citations in OpenAlex.
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Review
- KI04 an Aminoglycosides-Derived Molecule Acts as an Inhibitor of Human Connexin46 Hemichannels Expressed in HeLa Cells.Biomolecules · 2023Article
- Intercellular Transmission of Hepatic ER Stress in Obesity Disrupts Systemic Metabolism.Cell metabolism · 2021Article
- Review
- Gap junctional signaling in pattern regulation: Physiological network connectivity instructs growth and form.Developmental neurobiology · 2017Review
- Communication of Ca(2+) signals via tunneling membrane nanotubes is mediated by transmission of inositol trisphosphate through gap junctions.Cell calcium · 2016Article
- Extracellular gentamicin reduces the activity of connexin hemichannels and interferes with purinergic Ca(2+) signaling in HeLa cells.Frontiers in cellular neuroscience · 2014Article
- Calcium flashes orchestrate the wound inflammatory response through DUOX activation and hydrogen peroxide release.Current biology : CB · 2013Article
- Gap junction communication in myelinating glia.Biochimica et biophysica acta · 2013Review
- Spatiotemporally controlled single cell sonoporation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Manipulating connexin communication channels: use of peptidomimetics and the translational outputs.The Journal of membrane biology · 2012Review
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
- Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.Cell communication & adhesion · 2012Article
- Communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (P2) and glutamatergic receptors.PloS one · 2012Article
- Gap junction assembly: roles for the formation plaque and regulation by the C-terminus of connexin43.Molecular biology of the cell · 2012Article
- Investigation of the reciprocal relationship between the expression of two gap junction connexin proteins, connexin46 and connexin43.The Journal of biological chemistry · 2011Article
- Autophagy regulates endoplasmic reticulum homeostasis and calcium mobilization in T lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Low connexin channel-dependent intercellular communication in human adult hematopoietic progenitor/stem cells: probing mechanisms of autologous stem cell therapy.Cell communication & adhesion · 2009Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
This study was undertaken to obtain direct evidence for the involvement of gap junctions in the propagation of intercellular Ca(2+) waves. Gap junction-deficient HeLa cells were transfected with plasmids encoding for green fluorescent protein (GFP) fused to the cytoplasmic carboxyl termini of connexin 43 (Cx43), 32 (Cx32), or 26 (Cx26). The subsequently expressed GFP-labeled gap junctions rendered the cells dye- and electrically coupled and were detected at the plasma membranes at points of contact between adjacent cells. To correlate the distribution of gap junctions with the changes in [Ca(2+)](i) associated with Ca(2+) waves and the distribution of the endoplasmic reticulum (ER), cells were loaded with fluorescent Ca(2+)-sensitive (fluo-3 and fura-2) and ER membrane (ER-Tracker) dyes. Digital high-speed microscopy was used to collect a series of image slices from which the three-dimensional distribution of the gap junctions and ER were reconstructed. Subsequently, intercellular Ca(2+) waves were induced in these cells by mechanical stimulation with or without extracellular apyrase, an ATP-degrading enzyme. In untransfected HeLa cells and in the absence of apyrase, cell-to-cell propagating [Ca(2+)](i) changes were characterized by initiating Ca(2+) puffs associated with the perinuclear ER. By contrast, in Cx-GFP-transfected cells and in the presence of apyrase, [Ca(2+)](i) changes were propagated without initiating perinuclear Ca(2+) puffs and were communicated between cells at the sites of the Cx-GFP gap junctions. The efficiency of Cx expression determined the extent of Ca(2+) wave propagation. These results demonstrate that intercellular Ca(2+) waves may be propagated simultaneously via an extracellular pathway and an intracellular pathway through gap junctions and that one form of communication may mask the other.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.