ArticleMolecular biology of the cell2000
The yeast heat shock transcription factor changes conformation in response to superoxide and temperature.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Does Inter-Organellar Proteostasis Impact Yeast Quality and Performance During Beer Fermentation?Frontiers in genetics · 2020Article
- Regulation of the heat shock transcription factor Hsf1 in fungi: implications for temperature-dependent virulence traits.FEMS yeast research · 2018Review
- Article
- Biology of the heat shock response and protein chaperones: budding yeast (Saccharomyces cerevisiae) as a model system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Review
- Association of constitutive hyperphosphorylation of Hsf1p with a defective ethanol stress response in Saccharomyces cerevisiae sake yeast strains.Applied and environmental microbiology · 2012Article
- Review
- Induction and transcriptional regulation of laccases in fungi.Current genomics · 2011Article
- Role of heat shock transcription factor in Saccharomyces cerevisiae oxidative stress response.Eukaryotic cell · 2007Article
- In the yeast heat shock response, Hsf1-directed induction of Hsp90 facilitates the activation of the Slt2 (Mpk1) mitogen-activated protein kinase required for cell integrity.Eukaryotic cell · 2007Article
- The natural osmolyte trehalose is a positive regulator of the heat-induced activity of yeast heat shock transcription factor.Molecular and cellular biology · 2007Article
- Could heat shock transcription factors function as hydrogen peroxide sensors in plants?Annals of botany · 2006Review
- Do mitochondria regulate the heat-shock response in Saccharomyces cerevisiae?Current genetics · 2005Article
- Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element.Molecular and cellular biology · 2004Article
- Complex regulation of the yeast heat shock transcription factor.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In vitro DNA-binding assays demonstrate that the heat shock transcription factor (HSF) from the yeast Saccharomyces cerevisiae can adopt an altered conformation when stressed. This conformation, reflected in a change in electrophoretic mobility, requires that two HSF trimers be bound to DNA. Single trimers do not show this change, which appears to represent an alteration in the cooperative interactions between trimers. HSF isolated from stressed cells displays a higher propensity to adopt this altered conformation. Purified HSF can be stimulated in vitro to undergo the conformational change by elevating the temperature or by exposing HSF to superoxide anion. Mutational analysis maps a region critical for this conformational change to the flexible loop between the minimal DNA-binding domain and the flexible linker that joins the DNA-binding domain to the trimerization domain. The significance of these findings is discussed in the context of the induction of the heat shock response by ischemic stroke, hypoxia, and recovery from anoxia, all known to stimulate the production of superoxide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.