ArticleMolecular biology of the cell2000
Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 112 citations in OpenAlex.
- Exploring roles for essential proteins in yeast filamentous growth identifies the WASP homolog Las17 as a regulator of the Cdc42-dependent fMAPK pathway.bioRxiv : the preprint server for biology · 2026Article
- The role of cell division control protein 42 in tumor and non-tumor diseases: A systematic review.Journal of Cancer · 2022Review
- Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- A class-II myosin is required for growth, conidiation, cell wall integrity and pathogenicity of Magnaporthe oryzae.Virulence · 2017Article
- Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis.PLoS biology · 2016Article
- Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Role of phosphatidylinositol phosphate signaling in the regulation of the filamentous-growth mitogen-activated protein kinase pathway.Eukaryotic cell · 2015Article
- The role of Sec3p in secretory vesicle targeting and exocyst complex assembly.Molecular biology of the cell · 2014Article
- Cdc1 removes the ethanolamine phosphate of the first mannose of GPI anchors and thereby facilitates the integration of GPI proteins into the yeast cell wall.Molecular biology of the cell · 2014Article
- Release from myosin V via regulated recruitment of an E3 ubiquitin ligase controls organelle localization.Developmental cell · 2014Article
- Asymmetric cell division in polyploid giant cancer cells and low eukaryotic cells.BioMed research international · 2014Review
- Cell wall-related bionumbers and bioestimates of Saccharomyces cerevisiae and Candida albicans.Eukaryotic cell · 2014Review
- Active segregation of yeast mitochondria by Myo2 is essential and mediated by Mmr1 and Ypt11.Current biology : CB · 2013Article
- A cost-benefit analysis of the physical mechanisms of membrane curvature.Nature cell biology · 2013Review
- Changes in cell morphology are coordinated with cell growth through the TORC1 pathway.Current biology : CB · 2013Article
- Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast.Molecular biology of the cell · 2012Article
- A link between mitotic entry and membrane growth suggests a novel model for cell size control.The Journal of cell biology · 2012Article
- Morphogenesis and the cell cycle.Genetics · 2012Review
- Cortical actin dynamics: Generating randomness by formin(g) and moving.Bioarchitecture · 2011Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We examined the role of the actin cytoskeleton in secretion in Saccharomyces cerevisiae with the use of several quantitative assays, including time-lapse video microscopy of cell surface growth in individual living cells. In latrunculin, which depolymerizes filamentous actin, cell surface growth was completely depolarized but still occurred, albeit at a reduced level. Thus, filamentous actin is necessary for polarized secretion but not for secretion per se. Consistent with this conclusion, latrunculin caused vesicles to accumulate at random positions throughout the cell. Cortical actin patches cluster at locations that correlate with sites of polarized secretion. However, we found that actin patch polarization is not necessary for polarized secretion because a mutant, bee1Delta(las17Delta), which completely lacks actin patch polarization, displayed polarized growth. In contrast, a mutant lacking actin cables, tpm1-2 tpm2Delta, had a severe defect in polarized growth. The yeast class V myosin Myo2p is hypothesized to mediate polarized secretion. A mutation in the motor domain of Myo2p, myo2-66, caused growth to be depolarized but with only a partial decrease in the level of overall growth. This effect is similar to that of latrunculin, suggesting that Myo2p interacts with filamentous actin. However, inhibition of Myo2p function by expression of its tail domain completely abolished growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.