ArticleMolecular biology of the cell2000
Truncation mutants of the tight junction protein ZO-1 disrupt corneal epithelial cell morphology.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Size-shrinking nanoparticles with high drug-protein payload for efficient, non-invasive treatment of corneal neovascularization.Journal of nanobiotechnology · 2026Article
- Novel Cell Culture Paradigm Prolongs Mouse Corneal Epithelial Cell Proliferative ActivityFrontiers in cell and developmental biology · 2021Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Epithelialization of mouse ovarian tumor cells originating in the fallopian tube stroma.Oncotarget · 2016Article
- MT2-MMP induces proteolysis and leads to EMT in carcinomas.Oncotarget · 2016Article
- Tight Junction Protein 1a regulates pigment cell organisation during zebrafish colour patterning.eLife · 2015Article
- Diadenosine tetraphosphate induces tight junction disassembly thus increasing corneal epithelial permeability.British journal of pharmacology · 2015Article
- Article
- Family-wide investigation of PDZ domain-mediated protein-protein interactions implicates β-catenin in maintaining the integrity of tight junctions.Chemistry & biology · 2013Article
- Article
- Targeted disruption of core 1 β1,3-galactosyltransferase (C1galt1) induces apical endocytic trafficking in human corneal keratinocytes.PloS one · 2012Article
- Connexin 43 connexon to gap junction transition is regulated by zonula occludens-1.Molecular biology of the cell · 2011Article
- Cdc42-dependent formation of the ZO-1/MRCKβ complex at the leading edge controls cell migration.The EMBO journal · 2011Article
- ZO-1 determines adherens and gap junction localization at intercalated disks.American journal of physiology. Heart and circulatory physiology · 2011Article
- Elevated pressure downregulates ZO-1 expression and disrupts cytoskeleton and focal adhesion in human trabecular meshwork cells.Molecular vision · 2011Article
- Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier.The Journal of biological chemistry · 2009Article
- NC-1059: a channel-forming peptide that modulates drug delivery across in vitro corneal epithelium.Investigative ophthalmology & visual science · 2009Article
- JunD represses transcription and translation of the tight junction protein zona occludens-1 modulating intestinal epithelial barrier function.Molecular biology of the cell · 2008Article
- Pcbs and tight junction expression.Environmental toxicology and pharmacology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tight junction is the most apical intercellular junction of epithelial cells and regulates transepithelial permeability through the paracellular pathway. To examine possible functions for the tight junction-associated protein ZO-1, C-terminally truncated mutants and a deletion mutant of ZO-1 were epitope tagged and stably expressed in corneal epithelial cell lines. Only full-length ZO-1 and one N-terminal truncation mutant targeted to cell borders; other mutants showed variable cytoplasmic distributions. None of the mutants initially disrupted the localization of endogenous ZO-1. However, long-term stable expression of two of the N-terminal mutants resulted in a dramatic change in cell shape and patterns of gene expression. An elongated fibroblast-like shape replaced characteristic epithelial cobblestone morphology. In addition, vimentin and smooth muscle actin expression were up-regulated, although variable cytokeratin expression remained, suggesting a partial transformation to a mesenchymal cell type. Concomitant with the morphological change, the expression of the integral membrane tight junction protein occludin was significantly down-regulated. The localizations of endogenous ZO-1 and another family member, ZO-2, were disrupted. These findings suggest that ZO-1 may participate in regulation of cellular differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.