ArticleMolecular biology of the cell2000
Activating phosphorylation of the Saccharomyces cerevisiae cyclin-dependent kinase, cdc28p, precedes cyclin binding.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- CDK Regulation of Meiosis: Lessons fromGenes · 2020Review
- The Neurospora RNA polymerase II kinase CTK negatively regulates catalase expression in a chromatin context-dependent manner.Environmental microbiology · 2020Article
- A comprehensive, mechanistically detailed, and executable model of the cell division cycle in Saccharomyces cerevisiae.Nature communications · 2019Article
- Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality.Oncogene · 2019Article
- Phosphoproteome dynamics during mitotic exit in budding yeast.The EMBO journal · 2018Article
- Ser or Leu: structural snapshots of mistranslation in Candida albicans.Frontiers in molecular biosciences · 2014Review
- The CDK Network: Linking Cycles of Cell Division and Gene Expression.Genes & cancer · 2012Article
- An overview of Cdk1-controlled targets and processes.Cell division · 2010Article
- A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1.Gene · 2009Article
- Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase.Cell division · 2009Article
- Functional evolution of cyclin-dependent kinases.Molecular biotechnology · 2009Review
- Distinct activation pathways confer cyclin-binding specificity on Cdk1 and Cdk2 in human cells.Molecular cell · 2008Article
- Article
- Bayesian Orthogonal Least Squares (BOLS) algorithm for reverse engineering of gene regulatory networks.BMC bioinformatics · 2007Article
- The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Trypanosoma brucei.Journal of molecular biology · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Phosphorylation by Cak1 regulates the C-terminal domain kinase Ctk1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.The Plant cell · 2004Article
- Regulation by phosphorylation of Pho81p, a cyclin-dependent kinase inhibitor in Saccharomyces cerevisiae.Current genetics · 2004Article
- The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p.Molecular and cellular biology · 2003Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic cell cycle progression is controlled by a family of protein kinases known as cyclin-dependent kinases (Cdks). Two steps are essential for Cdk activation: binding of a cyclin and phosphorylation on a conserved threonine residue by the Cdk-activating kinase (CAK). We have studied the interplay between these regulatory mechanisms during the activation of the major Saccharomyces cerevisiae Cdk, Cdc28p. We found that the majority of Cdc28p was phosphorylated on its activating threonine (Thr-169) throughout the cell cycle. The extent of Thr-169 phosphorylation was similar for monomeric Cdc28p and Cdc28p bound to cyclin. By varying the order of the addition of cyclin and Cak1p, we determined that Cdc28p was activated most efficiently when it was phosphorylated before cyclin binding. Furthermore, we found that a Cdc28p(T169A) mutant, which cannot be phosphorylated, bound cyclin less well than wild-type Cdc28p in vivo. These results suggest that unphosphorylated Cdc28p may be unable to bind tightly to cyclin. We propose that Cdc28p is normally phosphorylated by Cak1p before it binds cyclin. This activation pathway contrasts with that in higher eukaryotes, in which cyclin binding appears to precede activating phosphorylation.
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