ArticleProceedings of the National Academy of Sciences of the United States of America2000
Rapid regulated dense-core vesicle exocytosis requires the CAPS protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 93 citations in OpenAlex.
- Neurons and molecules involved in noxious light sensation in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses.PLoS biology · 2025Article
- Review
- Glial-derived mitochondrial signals affect neuronal proteostasis and aging.Science advances · 2023Article
- PI(4,5)P2-dependent and -independent roles of PI4P in the control of hormone secretion by pituitary cells.Frontiers in endocrinology · 2023Review
- RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission.Journal of neurochemistry · 2021Article
- Synapsin Is Required for Dense Core Vesicle Capture and cAMP-Dependent Neuropeptide Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- (M)Unc13s in Active Zone Diversity: AFrontiers in synaptic neuroscience · 2021Review
- Exocytotic fusion pore under stress.Cell stress · 2020Review
- CAPS-1 requires its C2, PH, MHD1 and DCV domains for dense core vesicle exocytosis in mammalian CNS neurons.Scientific reports · 2017Article
- Ketamine Inhibits ATP-Evoked Exocytotic Release of Brain-Derived Neurotrophic Factor from Vesicles in Cultured Rat Astrocytes.Molecular neurobiology · 2016Article
- Astrocytes as secretory cells of the central nervous system: idiosyncrasies of vesicular secretion.The EMBO journal · 2016Review
- Excitable Astrocytes: Ca(2+)- and cAMP-Regulated Exocytosis.Neurochemical research · 2015Review
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.Nature protocols · 2013Article
- Article
- The Ca(2+)-dependent activator protein for secretion CAPS: do I dock or do I prime?Molecular neurobiology · 2009Review
- CAPS facilitates filling of the rapidly releasable pool of large dense-core vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Sdmg1 is a conserved transmembrane protein associated with germ cell sex determination and germline-soma interactions in mice.Development (Cambridge, England) · 2008Article
- Tomosyn negatively regulates CAPS-dependent peptide release at Caenorhabditis elegans synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Although many proteins essential for regulated neurotransmitter and peptide hormone secretion have been identified, little is understood about their precise roles at specific stages of the multistep pathway of exocytosis. To study the function of CAPS (Ca(2+)-dependent activator protein for secretion), a protein required for Ca(2+)-dependent exocytosis of dense-core vesicles, secretory responses in single rat melanotrophs were monitored by patch-clamp membrane capacitance measurements. Flash photolysis of caged Ca(2+) elicited biphasic capacitance increases consisting of rapid and slow components with distinct Ca(2+) dependencies. A threshold of approximately 10 microM Ca(2+) was required to trigger the slow component, while the rapid capacitance increase was recorded already at a intracellular Ca(2+) activity < 10 microM. Both kinetic membrane capacitance components were abolished by botulinum neurotoxin B or E treatment, suggesting involvement of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-dependent vesicle fusion. The rapid but not the slow component was inhibited by CAPS antibody. These results were further clarified by immunocytochemical studies that revealed that CAPS was present on only a subset of dense-core vesicles. Overall, the results indicate that dense-core vesicle exocytosis in melanotrophs occurs by two parallel pathways. The faster pathway exhibits high sensitivity to Ca(2+) and requires the presence of CAPS, which appears to act at a late stage in the secretory pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.