ArticleProceedings of the National Academy of Sciences of the United States of America2000
Epithelial and endothelial expression of the green fluorescent protein reporter gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 73 citations in OpenAlex.
- Cellular Prion Protein as a Novel Regulator of Vascular Plexus Stability During Retinal Angiogenesis.Neurochemical research · 2026Article
- Unlocking Genetic Mysteries during the Epic Sperm Journey toward Fertilization: Further ExpandingBiomolecules · 2024Review
- Article
- Role of hypoxia‑mediated cellular prion protein functional change in stem cells and potential application in angiogenesis (Review).Molecular medicine reports · 2017Article
- PrPC expression and prion seeding activity in the alimentary tract and lymphoid tissue of deer.PloS one · 2017Article
- Translation of the prion protein mRNA is robust in astrocytes but does not amplify during reactive astrocytosis in the mouse brain.PloS one · 2014Article
- Roles of the cellular prion protein in the regulation of cell-cell junctions and barrier function.Tissue barriers · 2013Review
- Molecular chaperone mediated late-stage neuroprotection in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.PloS one · 2013Article
- Suppression of protein aggregation by chaperone modification of high molecular weight complexes.Brain : a journal of neurology · 2012Article
- Expression patterns of prion protein gene in differential genotypes sheep: quantification using molecular beacon real-time RT-PCR.Virus genes · 2011Article
- Dramatic reduction of PrP C level and glycosylation in peripheral nerves following PrP knock-out from Schwann cells does not prevent transmissible spongiform encephalopathy neuroinvasion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Neuronal SMN expression corrects spinal muscular atrophy in severe SMA mice while muscle-specific SMN expression has no phenotypic effect.Human molecular genetics · 2008Article
- Developmental expression of PrP in the post-implantation embryo.Brain research · 2007Article
- Overexpression of PrPC by adenovirus-mediated gene targeting reduces ischemic injury in a stroke rat model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer.Mammalian genome : official journal of the International Mammalian Genome Society · 2003Article
- M129V variation in the prion protein may influence cognitive performance.Molecular psychiatry · 2003Article
- Filamentous tau in oligodendrocytes and astrocytes of transgenic mice expressing the human tau isoform with the P301L mutation.The American journal of pathology · 2003Article
- Disease-associated prion protein in vessel walls.The American journal of pathology · 2002Article
- Transgenesis applied to transmissible spongiform encephalopathies.Transgenic research · 2002Review
- PrP expression and replication by Schwann cells: implications in prion spreading.Journal of virology · 2002Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the cellular form of the prion protein (PrP(c)) gene is required for prion replication and neuroinvasion in transmissible spongiform encephalopathies. The identification of the cell types expressing PrP(c) is necessary to understanding how the agent replicates and spreads from peripheral sites to the central nervous system. To determine the nature of the cell types expressing PrP(c), a green fluorescent protein reporter gene was expressed in transgenic mice under the control of 6.9 kb of the bovine PrP gene regulatory sequences. It was shown that the bovine PrP gene is expressed as two populations of mRNA differing by alternative splicing of one 115-bp 5' untranslated exon in 17 different bovine tissues. The analysis of transgenic mice showed reporter gene expression in some cells that have been identified as expressing PrP, such as cerebellar Purkinje cells, lymphocytes, and keratinocytes. In addition, expression of green fluorescent protein was observed in the plexus of the enteric nervous system and in a restricted subset of cells not yet clearly identified as expressing PrP: the epithelial cells of the thymic medullary and the endothelial cells of both the mucosal capillaries of the intestine and the renal capillaries. These data provide valuable information on the distribution of PrP(c) at the cellular level and argue for roles of the epithelial and endothelial cells in the spread of infection from the periphery to the brain. Moreover, the transgenic mice described in this paper provide a model that will allow for the study of the transcriptional activity of the PrP gene promoter in response to scrapie infection.
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