ArticleThe Journal of cell biology2000
Electromechanical coupling between skeletal and cardiac muscle. Implications for infarct repair.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed, 1 synthesis or guideline pooled it, 347 citations in OpenAlex.
- Induced pluripotent stem cell therapies in heart failure treatment: a meta-analysis and systematic review.Regenerative medicinePooled it
- A Review of the Therapeutic Efficacy and Safety of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Preclinical Models of Subacute and Chronic Myocardial Infarction.Journal of cardiovascular development and disease · 2026Review
- Decellularized extracellular matrix materials for treatment of ischemic cardiomyopathy.Bioactive materials · 2024Review
- The sinoatrial node extracellular matrix promotes pacemaker phenotype and protects automaticity in engineered heart tissues from cyclic strain.Cell reports · 2023Article
- Remodeled eX vivo muscle engineered tissue improves heart function after chronic myocardial ischemia.Scientific reports · 2023Article
- Healing the Ischaemic Heart: A Critical Review of Stem Cell Therapies.Reviews in cardiovascular medicine · 2023Review
- Arrhythmogenic risks of stem cell replacement therapy for cardiovascular diseases.Journal of cellular physiology · 2020Review
- Function Follows Form - A Review of Cardiac Cell Therapy.Circulation journal : official journal of the Japanese Circulation Society · 2019Review
- Long-Term Engraftment (16 Years) of Myoblasts in a Human Infarcted Heart.Stem cells translational medicine · 2018Article
- Clinical Studies of Cell Therapy in Cardiovascular Medicine: Recent Developments and Future Directions.Circulation research · 2018Review
- Can We Engineer a Human Cardiac Patch for Therapy?Circulation research · 2018Review
- Translating Myocardial Remuscularization.Circulation research · 2017Review
- Cardiac progenitor cells application in cardiovascular disease.Journal of cardiovascular and thoracic research · 2017Review
- Translational cardiac stem cell therapy: advancing from first-generation to next-generation cell types.NPJ Regenerative medicine · 2017Review
- Cell Therapies in Cardiomyopathy: Current Status of Clinical Trials.Analytical cellular pathology (Amsterdam) · 2017Review
- Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails.Cell research · 2015Article
- Arrhythmia in stem cell transplantation.Cardiac electrophysiology clinics · 2015Review
- Exogenous connexin43-expressing autologous skeletal myoblasts ameliorate mechanical function and electrical activity of the rabbit heart after experimental infarction.International journal of experimental pathology · 2015Article
- Cardiac regeneration using pluripotent stem cells--progression to large animal models.Stem cell research · 2014Review
- Molecular imaging, biodistribution and efficacy of mesenchymal bone marrow cell therapy in a mouse model of Chagas disease.Microbes and infection · 2014Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Skeletal myoblasts form grafts of mature muscle in injured hearts, and these grafts contract when exogenously stimulated. It is not known, however, whether cardiac muscle can form electromechanical junctions with skeletal muscle and induce its synchronous contraction. Here, we report that undifferentiated rat skeletal myoblasts expressed N-cadherin and connexin43, major adhesion and gap junction proteins of the intercalated disk, yet both proteins were markedly downregulated after differentiation into myo-tubes. Similarly, differentiated skeletal muscle grafts in injured hearts had no detectable N-cadherin or connexin43; hence, electromechanical coupling did not occur after in vivo grafting. In contrast, when neonatal or adult cardiomyocytes were cocultured with skeletal muscle, approximately 10% of the skeletal myotubes contracted in synchrony with adjacent cardiomyocytes. Isoproterenol increased myotube contraction rates by 25% in coculture without affecting myotubes in monoculture, indicating the cardiomyocytes were the pacemakers. The gap junction inhibitor heptanol aborted myotube contractions but left spontaneous contractions of individual cardiomyocytes intact, suggesting myotubes were activated via gap junctions. Confocal microscopy revealed the expression of cadherin and connexin43 at junctions between myotubes and neonatal or adult cardiomyocytes in vitro. After microinjection, myotubes transferred dye to neonatal cardiomyocytes via gap junctions. Calcium imaging revealed synchronous calcium transients in cardiomyocytes and myotubes. Thus, cardiomyocytes can form electromechanical junctions with some skeletal myotubes in coculture and induce their synchronous contraction via gap junctions. Although the mechanism remains to be determined, if similar junctions could be induced in vivo, they might be sufficient to make skeletal muscle grafts beat synchronously with host myocardium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.