ArticleThe Journal of cell biology2000
Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Efficient termination of transcription by RNA polymerase I requires a conserved hairpin of the ribosomal RNA precursor.Science advances · 2025Article
- H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation.Nature communications · 2025Article
- The Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5' rRNA domain of an early 90S pre-ribosomal particle.Nucleic acids research · 2024Article
- Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release.Cell reports · 2022Article
- Production of nascent ribosome precursors within the nucleolar microenvironment of Saccharomyces cerevisiae.Genetics · 2022Article
- Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.EMBO reports · 2021Article
- Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.The Journal of biological chemistry · 2019Article
- Article
- Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- RNA polymerase I stability couples cellular growth to metal availability.Molecular cell · 2013Article
- Regulation of ribosomal RNA production by RNA polymerase I: does elongation come first?Genetics research international · 2012Article
- Transcriptional repressor NIR functions in the ribosome RNA processing of both 40S and 60S subunits.PloS one · 2012Article
- Powering through ribosome assembly.RNA (New York, N.Y.) · 2009Review
- A novel small-subunit processome assembly intermediate that contains the U3 snoRNP, nucleolin, RRP5, and DBP4.Molecular and cellular biology · 2009Article
- Transcription regulation of the rRNA gene by a multifunctional nucleolar protein, B23/nucleophosmin, through its histone chaperone activity.Molecular and cellular biology · 2008Article
- Function and assembly of a chromatin-associated RNase P that is required for efficient transcription by RNA polymerase I.PloS one · 2008Article
- Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis.Molecular and cellular biology · 2007Article
- Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.Molecular biology of the cell · 2007Article
- A cotranscriptional model for 3'-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor.RNA (New York, N.Y.) · 2004Article
- A plant snoRNP complex containing snoRNAs, fibrillarin, and nucleolin-like proteins is competent for both rRNA gene binding and pre-rRNA processing in vitro.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel ribonucleoprotein complex enriched in nucleolar proteins was purified from yeast extracts and constituents were identified by mass spectrometry. When isolated from rapidly growing cells, the assembly contained ribonucleic acid (RNA) polymerase (pol) I, and some of its transcription factors like TATA-binding protein (TBP), Rrn3p, Rrn5p, Rrn7p, and Reb1p along with rRNA processing factors, like Nop1p, Cbf5p, Nhp2p, and Rrp5p. The small nucleolar RNAs (snoRNAs) U3, U14, and MRP were also found to be associated with the complex, which supports accurate transcription, termination, and pseudouridylation of rRNA. Formation of the complex did not depend on pol I, and the complex could efficiently recruit exogenous pol I into active ribosomal DNA (rDNA) transcription units. Visualization of the complex by electron microscopy and immunogold labeling revealed a characteristic cluster-forming network of nonuniform size containing nucleolar proteins like Nop1p and Fpr3p and attached pol I. Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing.
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