ArticleThe Journal of cell biology2000
Partitioning of the 2-microm circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded rep protein distribution.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 66 citations in OpenAlex.
- pSB3, a member of the 2-micron family of plasmids, replicates and is efficiently partitioned in multiple budding yeast species.G3 (Bethesda, Md.) · 2026Article
- The selfish yeast plasmid exploits a SWI/SNF-type chromatin remodeling complex for hitchhiking on chromosomes and ensuring high-fidelity propagation.PLoS genetics · 2023Article
- The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function.Nucleic acids research · 2022Article
- The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.PLoS genetics · 2021Article
- A natural variant of the essential host geneeLife · 2020Article
- DNA sequence elements required for partitioning competence of the Saccharomyces cerevisiae 2-micron plasmid STB locus.Nucleic acids research · 2019Article
- The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae.Current genetics · 2018Review
- The possible function of Flp1 in homologous recombination repair inAIMS genetics · 2018Article
- The yeast 2-μm plasmid Raf protein contributes to plasmid inheritance by stabilizing the Rep1 and Rep2 partitioning proteins.Nucleic acids research · 2017Article
- The 2-μm plasmid encoded protein Raf1 regulates both stability and copy number of the plasmid by blocking the formation of the Rep1-Rep2 repressor complex.Nucleic acids research · 2017Article
- Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome.Nucleic acids research · 2016Article
- The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells.Microbiology spectrum · 2014Review
- A selfish DNA element engages a meiosis-specific motor and telomeres for germ-line propagation.The Journal of cell biology · 2014Article
- Article
- Co-segregation of yeast plasmid sisters under monopolin-directed mitosis suggests association of plasmid sisters with sister chromatids.Nucleic acids research · 2013Article
- Temporal sequence and cell cycle cues in the assembly of host factors at the yeast 2 micron plasmid partitioning locus.Nucleic acids research · 2013Article
- Article
- New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.G3 (Bethesda, Md.) · 2012Article
- CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast.The Journal of cell biology · 2011Article
- Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromere.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The efficient partitioning of the 2-microm plasmid of Saccharomyces cerevisiae at cell division is dependent on two plasmid-encoded proteins (Rep1p and Rep2p), together with the cis-acting locus REP3 (STB). In addition, host encoded factors are likely to contribute to plasmid segregation. Direct observation of a 2-microm-derived plasmid in live yeast cells indicates that the multiple plasmid copies are located in the nucleus, predominantly in clusters with characteristic shapes. Comparison to a single-tagged chromosome or to a yeast centromeric plasmid shows that the segregation kinetics of the 2-microm plasmid and the chromosome are quite similar during the yeast cell cycle. Immunofluorescence analysis reveals that the plasmid is colocalized with the Rep1 and Rep2 proteins within the yeast nucleus. Furthermore, the Rep proteins (and therefore the plasmid) tend to concentrate near the poles of the yeast mitotic spindle. Depolymerization of the spindle results in partial dispersion of the Rep proteins in the nucleus concomitant with a loosening in the association between plasmid molecules. In an ipl1-2 yeast strain, shifted to the nonpermissive temperature, the chromosomes and plasmid almost always missegregate in tandem. Our results suggest that, after DNA replication, plasmid distribution to the daughter cells occurs in the form of specific DNA-protein aggregates. They further indicate that the plasmid partitioning mechanism may exploit at least some of the components of the cellular machinery required for chromosomal segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.