ArticleThe EMBO journal2000
DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 191 citations in OpenAlex.
- Structural Activation of DNA Unwinding by MCM8/9/HROB.bioRxiv : the preprint server for biology · 2026Article
- Cohesin complex oligomerization maintains end-tethering at DNA double-strand breaks.Nature cell biology · 2025Article
- CMG helicase disassembly is essential and driven by two pathways in budding yeast.The EMBO journal · 2024Article
- Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoa.Cell · 2024Article
- High abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.Cell proliferation · 2022Article
- Cohesin regulates homology search during recombinational DNA repair.Nature cell biology · 2021Article
- Identification and analysis of genes associated with epithelial ovarian cancer by integrated bioinformatics methods.PloS one · 2021Article
- Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction.International journal of molecular sciences · 2020Article
- Cohesin Causes Replicative DNA Damage by Trapping DNA Topological Stress.Molecular cell · 2020Article
- Article
- DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation.Molecular cell · 2018Article
- SMC complexes differentially compact mitotic chromosomes according to genomic context.Nature cell biology · 2017Article
- Recruitment of Fkh1 to replication origins requires precisely positioned Fkh1/2 binding sites and concurrent assembly of the pre-replicative complex.PLoS genetics · 2017Article
- Chromosome Duplication in Saccharomyces cerevisiae.Genetics · 2016Review
- Structure of human Cdc45 and implications for CMG helicase function.Nature communications · 2016Article
- Pathogenic Network Analysis Predicts Candidate Genes for Cervical Cancer.Computational and mathematical methods in medicine · 2016Article
- The Sulfolobus solfataricus GINS Complex Stimulates DNA Binding and Processive DNA Unwinding by Minichromosome Maintenance Helicase.Journal of bacteriology · 2015Article
- Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos.Cell reports · 2015Article
- Checkpoint-dependent RNR induction promotes fork restart after replicative stress.Scientific reports · 2015Article
- Archaeal MCM Proteins as an Analog for the Eukaryotic Mcm2-7 Helicase to Reveal Essential Features of Structure and Function.Archaea (Vancouver, B.C.) · 2015Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc45p assembles at replication origins before initia tion and is required for origin firing in Saccharomyces cerevisiae. A heat-inducible cdc45 degron mutant was constructed that promotes rapid degradation of Cdc45p at the restrictive temperature. Consistent with a role in initiation, loss of Cdc45p in G(1) prevents all detectable DNA replication without preventing subsequent entry into mitosis. Loss of Cdc45p activity during S-phase blocks S-phase completion but not activation of replication checkpoints. Using density substitution, we show that after allowing replication fork establishment, Cdc45p inactivation prevents the subsequent progression of individual replication forks. This provides the first direct functional evidence that Cdc45p plays an essential role during elongation. Thus, like the large T antigen in SV40 replication, Cdc45p plays a central role in both initiation and elongation phases of chromosomal DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.