ArticleThe EMBO journal2000
TCRalpha enhancer activation occurs via a conformational change of a pre-assembled nucleo-protein complex.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- CREsted: modeling genomic and synthetic cell-type-specific enhancers across tissues and species.Nature methods · 2026Article
- The insulator EACBE regulates V(D)J recombination of Tcrd gene by modulating chromatin organization.Frontiers in immunology · 2025Article
- Enhancers in T Cell development and malignant lesions.Cell death discovery · 2024Review
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Blueprint of human thymopoiesis reveals molecular mechanisms of stage-specific TCR enhancer activation.The Journal of experimental medicine · 2020Article
- T-cell receptor α enhancer is inactivated in αβ T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Recent insights into the transcriptional control of the Tcra/Tcrd locus by distant enhancers during the development of T-lymphocytes.Transcription · 2015Review
- Article
- Unifying model for molecular determinants of the preselection Vβ repertoire.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Tcra enhancer activation by inducible transcription factors downstream of pre-TCR signaling.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Epigenetic repression of the Igk locus by STAT5-mediated recruitment of the histone methyltransferase Ezh2.Nature immunology · 2011Article
- H3K4 tri-methylation provides an epigenetic signature of active enhancers.The EMBO journal · 2011Article
- Transcription regulation from a TATA and INR-less promoter: spatial segregation of promoter function.The EMBO journal · 2006Article
- Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation.Molecular and cellular biology · 2003Article
- Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.Genes & development · 2002Article
- Control of IL-2Ralpha gene expression: structural changes within the proximal enhancer/core promoter during T-cell development.Nucleic acids research · 2002Article
- Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity.Oncogene · 2001Review
- Accessibility control of T cell receptor gene rearrangement in developing thymocytes. The TCR alpha/delta locus.Immunologic research · 2000Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TCR alpha enhancer (Ealpha) has served as a paradigm for studying how enhancers organize trans-activators into nucleo-protein complexes thought to recruit and synergistically stimulate the transcriptional machinery. Little is known, however, of either the extent or dynamics of Ealpha occupancy by nuclear factors during T cell development. Using dimethyl sulfate (DMS) in vivo footprinting, we demonstrate extensive Ealpha occupancy, encompassing both previously identified and novel sites, not only in T cells representing a developmental stage where Ealpha is known to be active (CD4(+)CD8(+)-DP cells), but surprisingly, also in cells at an earlier developmental stage where Ealpha is not active (CD4(-)CD8(-)-DN cells). Partial occupancy was also established in B-lymphoid but not non-lymphoid cells. In vivo DNase I footprinting, however, implied developmentally induced changes in nucleo-protein complex topography. Stage-specific differences in factor composition at Ealpha sequences were also suggested by EMSA analysis. These results, which indicate that alterations in the structure of a pre-assembled nucleo-protein complex correlate with the onset of Ealpha activity, may exemplify one mechanism by which enhancers can rapidly respond to incoming stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.