ArticleThe EMBO journal2000
Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 190 papers.
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Who cites it
190 citing papers in PubMed, 447 citations in OpenAlex.
- Effect of statins on clinical and molecular responses to intramuscular interferon beta-1a.Neurology · 2009Trial
- Computational insights on the interplay between electrotaxis and mechanotaxis.PLoS computational biology · 2026Article
- Fabrication of ECM protein coated hollow collagen channels to study peripheral nerve regeneration.Scientific reports · 2024Article
- A PAK family kinase and the Hippo/Yorkie pathway modulate WNT signaling to functionally integrate body axes during regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Phosphorylation of Arl4A/D promotes their binding by the HYPK chaperone for their stable recruitment to the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- MicroRNAs as the critical regulators of cell migration and invasion in thyroid cancer.Biomarker research · 2022Review
- Disturbed flow-induced FAK K152 SUMOylation initiates the formation of pro-inflammation positive feedback loop by inducing reactive oxygen species production in endothelial cells.Free radical biology & medicine · 2021Article
- Activation of neuronal Ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and axonal plasticity in mice.Journal of neurochemistry · 2021Article
- Actin flow-dependent and -independent force transmission through integrins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- NEDD4L-mediated Merlin ubiquitination facilitates Hippo pathway activation.EMBO reports · 2020Article
- Statins Disrupt Macrophage Rac1 Regulation Leading to Increased Atherosclerotic Plaque Calcification.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Mechanical Cues Affect Migration and Invasion of Cells From Three Different Directions.Frontiers in cell and developmental biology · 2020Review
- Effect of PAK Inhibition on Cell Mechanics Depends on Rac1.Frontiers in cell and developmental biology · 2020Article
- Activation of endothelial ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and angiogenesis via activating Pak1 in mice.Experimental neurology · 2019Review
- SHOC2 complex-driven RAF dimerization selectively contributes to ERK pathway dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Role of Lipid Rafts in Hematopoietic Stem Cells Homing, Mobilization, Hibernation, and Differentiation.Cells · 2019Review
- The Role of PAK1 in the Maturation of Invadopodia During Transient Mechanical Stimulation.Frontiers in cell and developmental biology · 2019Article
- Integrin and microtubule crosstalk in the regulation of cellular processes.Cellular and molecular life sciences : CMLS · 2018Review
- Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary.Scientific reports · 2018Article
- Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET.Nature chemical biology · 2018Article
130 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The small GTPase Rac regulates cytoskeletal organization, cell cycle progression, gene expression and oncogenic transformation, processes that depend upon both soluble growth factors and adhesion to the extracellular matrix (ECM). We now show that growth factors and adhesion to the ECM both contribute independently and approximately equally to Rac activation. However, activated Rac in non-adherent cells failed to stimulate the Rac effector PAK. V12 Rac or Rac activated by serum translocated to the membrane fraction of adherent cells but remained mainly cytoplasmic in suspended cells. An activated Rac mutant lacking a membrane-targeting sequence did not activate PAK in adherent cells, while mutations that forced membrane targeting restored PAK activation in suspended cells. In vitro, V12 Rac showed greater binding to membranes from adherent relative to suspended cells, indicating that cell adhesion regulated membrane binding sites for Rac. These results show that ECM regulates the ability of Rac to couple with PAK via an effect on membrane binding sites that facilitate their interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.