ArticleThe Journal of physiology2000
Endothelin-1 and photoreleased diacylglycerol increase L-type Ca2+ current by activation of protein kinase C in rat ventricular myocytes.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- αeLife · 2023Article
- IPAmerican journal of physiology. Heart and circulatory physiology · 2021Article
- A new stone for a new path, from "physiology to the bedside".Pflugers Archiv : European journal of physiology · 2019Article
- Hypertrophy of human embryonic stem cell-derived cardiomyocytes supported by positive feedback between CaPflugers Archiv : European journal of physiology · 2019Article
- DREADD technology reveals major impact of Gq signalling on cardiac electrophysiology.Cardiovascular research · 2019Article
- Physiologic, Pathologic, and Therapeutic Paracrine Modulation of Cardiac Excitation-Contraction Coupling.Circulation research · 2018Review
- Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC).PloS one · 2014Article
- Calcium and IP3 dynamics in cardiac myocytes: experimental and computational perspectives and approaches.Frontiers in pharmacology · 2014Review
- PKC independent inhibition of voltage gated calcium channels by volatile anesthetics in freshly isolated vascular myocytes from the aorta.Cell calcium · 2013Article
- 'Eventless' InsP3-dependent SR-Ca2+ release affecting atrial Ca2+ sparks.The Journal of physiology · 2013Article
- Endothelin-1 stimulates the expression of L-type Ca2+ channels in neonatal rat cardiomyocytes via the extracellular signal-regulated kinase 1/2 pathway.The Journal of membrane biology · 2013Article
- The role of the paracrine/autocrine mediator endothelin-1 in regulation of cardiac contractility and growth.British journal of pharmacology · 2013Review
- PKC-ε mediates multiple endothelin-1 actions on systolic Ca2+ and contractility in ventricular myocytes.Biochemical and biophysical research communications · 2012Article
- Kinase-dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes during chronic hypoxia.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Regulation of cardiac excitability by protein kinase C isozymes.Frontiers in bioscience (Scholar edition) · 2012Review
- Endothelin-1 augments Na⁺/H⁺ exchange activity in murine pulmonary arterial smooth muscle cells via Rho kinase.PloS one · 2012Article
- The cardiovascular physiology and pharmacology of endothelin-1.Advances in pharmacology (San Diego, Calif.) · 2010Review
- Protein kinase C isoforms differentially phosphorylate Ca(v)1.2 alpha(1c).Biochemistry · 2009Article
- Supramolecular assemblies and localized regulation of voltage-gated ion channels.Physiological reviews · 2009Review
- Endothelin-1 regulates cardiac L-type calcium channels via NAD(P)H oxidase-derived superoxide.The Journal of pharmacology and experimental therapeutics · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The amphotericin B-perforated whole-cell patch clamp technique was used to determine the modulation of L-type Ca2+ channels by protein kinase C (PKC)-mediated pathways in adult rat ventricular myocytes. Application of 10 nM endothelin-1 (ET-1) increased peak Ca2+ current (ICa) by 28.2 +/- 2.5 % (n = 13) and slowed current decay. These effects were prevented by the endothelin receptor antagonist PD145065 (10 microM) and by the PKC inhibitor chelerythrine (8 microM). To establish if direct activation of PKC mimicked the ET-1 effect, the active and inactive phorbol esters (phorbol-12-myristate-13-acetate and 4alpha-phorbol-12, 13-didecanoate) were tested. Both phorbol esters (100 nM) resulted in a small (approximately 10%) increase in ICa, suggesting PKC-independent effects. Bath application of dioctanoylglycerol (diC8), a diacylglycerol (DAG) analogue which is capable of directly activating PKC, caused a gradual decline in peak ICa (50.4 +/- 6.2 %, n = 5) and increased the rate of current decay. These effects were unaffected by the PKC inhibitor chelerythrine (8 microM). Intracellular photorelease of caged diC8 with 3 or 10 s exposure to UV light produced a concentration-dependent increase in peak ICa (20. 7 +/- 8.5 % (n = 8) for 3 s UV and 60.8 +/- 11.4 % (n = 13) for 10 s UV), which could be inhibited by chelerythrine. Our results demonstrate that both ET-1 and intracellularly photoreleased diC8 increase ICa by a PKC-mediated pathway, which is in direct contrast to the PKC-independent inhibition of ICa produced by bath-applied diC8. We conclude that specific cellular pools of DAG are crucially important in the regulation of ICa by PKC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.