ArticleJournal of clinical microbiology2000
Application of pulsed-field gel electrophoresis and binary typing as tools in veterinary clinical microbiology and molecular epidemiologic analysis of bovine and human Staphylococcus aureus isolates.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 152 citations in OpenAlex.
- Staphylococcal mastitis in dairy cows.Frontiers in veterinary science · 2024Review
- Study of antibiotics sensitivity pattern and molecular characterization of Staphylococcus aureus isolated from human and animal pyogenic cases.Archives of microbiology · 2022Article
- Article
- Risk Factors for the Occurrence of Methicillin-ResistantFoodborne pathogens and disease · 2020Review
- In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production ofInfection and drug resistance · 2020Article
- Technological interventions and advances in the diagnosis of intramammary infections in animals with emphasis on bovine population-a review.The veterinary quarterly · 2019Review
- Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Molecular typing of3 Biotech · 2018Article
- Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia.BMC research notes · 2017Article
- Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR).Journal of visualized experiments : JoVE · 2016Article
- The Application of Pulsed Field Gel Electrophoresis in Clinical Studies.Journal of clinical and diagnostic research : JCDR · 2016Review
- Associations between bacterial genotype and outcome of bovine clinical Staphylococcus aureus mastitis.Acta veterinaria Scandinavica · 2014Article
- Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, southern Iran.Iranian journal of veterinary research · 2014Article
- Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows.International journal of molecular sciences · 2013Article
- Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan.BMC veterinary research · 2012Article
- Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans.Journal of mammary gland biology and neoplasia · 2011Review
- Genotypic Characterization of Staphylococcus aureus Obtained from Humans and Bovine Mastitis Samples in India.Journal of global infectious diseases · 2011Article
- Studies with bioengineered Nisin peptides highlight the broad-spectrum potency of Nisin V.Microbial biotechnology · 2010Article
- Changing trends in mastitis.Irish veterinary journal · 2009Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thirty-eight bovine mammary Staphylococcus aureus isolates from diverse clinical, temporal, and geographical origins were genotyped by pulsed-field gel electrophoresis (PFGE) after SmaI digestion of prokaryotic DNA and by means of binary typing using 15 strain-specific DNA probes. Seven pulsed-field types and four subtypes were identified, as were 16 binary types. Concordant delineation of genetic relatedness was documented by both techniques, yet based on practical and epidemiological considerations, binary typing was the preferable method. Genotypes of bovine isolates were compared to 55 previously characterized human S. aureus isolates through cluster analysis of binary types. Genetic clusters containing strains of both human and bovine origin were found, but bacterial genotypes were predominantly associated with a single host species. Binary typing proved an excellent tool for comparison of S. aureus strains, including methicillin-resistant S. aureus, derived from different host species and from different databases. For 28 bovine S. aureus isolates, detailed clinical observations in vivo were compared to strain typing results in vitro. Associations were found between distinct genotypes and severity of disease, suggesting strain-specific bacterial virulence. Circumstantial evidence furthermore supports strain-specific routes of bacterial dissemination. We conclude that PFGE and binary typing can be successfully applied for genetic analysis of S. aureus isolates from bovine mammary secretions. Binary typing in particular is a robust and simple method and promises to become a powerful tool for strain characterization, for resolution of clonal relationships of bacteria within and between host species, and for identification of sources and transmission routes of bovine S. aureus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.