ArticleJournal of clinical microbiology2000
Evaluation of the LiPA MYCOBACTERIA assay for identification of mycobacterial species from BACTEC 12B bottles.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Revisiting the methods for detectingAccess microbiology · 2021Review
- Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species.Journal of clinical microbiology · 2010Article
- Species spectrum of nontuberculous mycobacteria isolated from clinical specimens in Kuwait.Current microbiology · 2008Article
- Use of a novel multiplex probe array for rapid identification of Mycobacterium species from clinical isolates.World journal of microbiology & biotechnology · 2007Article
- Identification of mycobacteria by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.Journal of clinical microbiology · 2006Article
- Molecular diagnostics in tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2005Review
- Prospective evaluation of the GenoType assay for routine identification of mycobacteria.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Article
- Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and genotype Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with Mycobacterial strains.Journal of clinical microbiology · 2004Article
- Mycobacterium reverse hybridization line-probe assay used to diagnose disseminated Mycobacterium haemophilum infection in a child with acute lymphoblastic leukemia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Article
- Novel mass spectrometry-based tool for genotypic identification of mycobacteria.Journal of clinical microbiology · 2004Article
- Detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacterial isolates by real-time PCR.Journal of clinical microbiology · 2003Article
- Evaluation of INNO-LiPA MYCOBACTERIA v2: improved reverse hybridization multiple DNA probe assay for mycobacterial identification.Journal of clinical microbiology · 2003Article
- Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes.Journal of clinical microbiology · 2003Article
- Novel allelic variants of Mycobacteria isolated in Brazil as determined by PCR-restriction enzyme analysis of hsp65.Journal of clinical microbiology · 2002Article
- Evaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.Journal of clinical microbiology · 2002Article
- GenoType mycobacterium assay for identification of mycobacterial species isolated from human clinical samples by using liquid medium.Journal of clinical microbiology · 2002Article
- Rapid identification of Mycobacteria to the species level using INNO-LiPA Mycobacteria, a reverse hybridization assay.Journal of clinical microbiology · 2001Article
- Direct identification of mycobacteria from MB/BacT alert 3D bottles: comparative evaluation of two commercial probe assays.Journal of clinical microbiology · 2001Article
- Performance assessment of new multiplex probe assay for identification of mycobacteria.Journal of clinical microbiology · 2001Article
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3 authors.
Funding
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Abstract
The LiPA MYCOBACTERIA (Innogenetics NV, Ghent, Belgium) assay was used to identify mycobacterial isolates using culture fluid from positive BACTEC 12B bottles. The LiPA method involves reverse hybridization of a biotinylated mycobacterial PCR fragment, a 16 to 23S rRNA spacer region, to oligonucleotide probes arranged in lines on a membrane strip, with detection via biotin-streptavidin coupling by a colorimetric system. This system identifies Mycobacterium species and differentiates M. tuberculosis complex, M. avium-M. intracellulare complex, and the following mycobacterial species: M. avium, M. intracellulare, M. kansasii, M. chelonae group, M. gordonae, M. xenopi, and M. scrofulaceum. The mycobacteria were identified in the laboratory by a series of tests, including the Roche AMPLICOR Mycobacterium tuberculosis (MTB) test, the Gen-Probe ACCUPROBE, and a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 65-kDa heat shock protein gene. The LiPA MYCOBACTERIA assay detected 60 mycobacterium isolates from 59 patients. There was complete agreement between LiPA and the laboratory identification tests for 26 M. tuberculosis complex, 9 M. avium, 3 M. intracellulare complex, 3 M. kansasii, 4 M. gordonae, and 5 M. chelonae group (all were M. abscessus) isolates. Three patient samples were LiPA positive for M. avium-M. intracellulare complex, and all were identified as M. intracellulare by the PCR-RFLP analysis. Seven additional mycobacterial species were LiPA positive for Mycobacterium spp. (six were M. fortuitum, and one was M. szulgai). The LiPA MYCOBACTERIA assay was easy to perform, and the interpretation of the positive bands was clear-cut. Following PCR amplification and gel electrophoresis, the LiPA assay was completed within 3 h.
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