ArticleJournal of clinical microbiology2000
Molecular differentiation of seven Malassezia species.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 122 citations in OpenAlex.
- First case of neonatal bloodstream infection caused byExperimental and therapeutic medicine · 2026Article
- Malassezia isolated from patients with PV exhibits resistance features supporting its pathogenic nature.Archives of microbiology · 2026Article
- Mechanism of the synergistic action of oxythiamine and ketoconazole against the yeast Malassezia pachydermatis.FEMS yeast research · 2026Article
- Molecular Diagnostics and Control of Zoonotic Dermatophytosis: First Detection ofAnimals : an open access journal from MDPI · 2025Article
- Automatic fluorescence microscopic image analyzer: a novel AI-based tool for early diagnosing superficial fungal infections.BMC infectious diseases · 2025Article
- Review
- Unveiling the structure of GPI-anchored protein of Malassezia globosa and its pathogenic role in pityriasis versicolor.Journal of molecular modeling · 2021Article
- Identification ofCurrent medical mycology · 2020Article
- In Vitro Azole and Amphotericin B Susceptibilities ofAntibiotics (Basel, Switzerland) · 2020Article
- Biochemical response of Rhodotorula mucilaginosa and Cladosporium herbarum isolated from aquatic environment on iron(III) ions.Scientific reports · 2019Article
- Geographical and Ethnic Differences Influence Culturable Commensal Yeast Diversity on Healthy Skin.Frontiers in microbiology · 2019Article
- Abundance and Species Diversity of Fungi in Rivers with Various Contaminations.Current microbiology · 2018Article
- Real-Time PCR Identification of Six Malassezia Species.Current microbiology · 2017Article
- Progress in Malassezia Research in Korea.Annals of dermatology · 2015Review
- Article
- Prolonged fever and splenic lesions caused by Malassezia restricta in an immunocompromised patient.Pediatric transplantation · 2014Article
- Identification of Malassezia Species Isolated from Patients with Pityriasis Versicolor Using PCR-RFLP Method in Markazi Province, Central Iran.Iranian journal of public health · 2014Article
- Article
- [Generation of reactive oxygen species in vitro by Malassezia yeasts].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2009Article
- Physiological and molecular characterization of atypical isolates of Malassezia furfur.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A system based on PCR and restriction endonuclease analysis was developed to distinguish the seven currently recognized Malassezia species. Seventy-eight strains, including authentic culture collection strains and routine clinical isolates, were investigated for variation in the ribosomal DNA repeat units. Two genomic regions, namely, the large subunit of the ribosomal gene and the internal transcribed spacer (ITS) region, were amplified by PCR, and products were digested with restriction endonucleases. The patterns generated were useful in identification of five out of seven Malassezia species. M. sympodialis was readily distinguishable in that its ITS region yielded a 700-bp amplified fragment, whereas the other six species yielded an 800-bp fragment. M. globosa and M. restricta were very similar in the regions studied and could be distinguished only by performing a hot start-touchdown PCR on primers for the beta-tubulin gene. Primers based on the conserved areas of the Candida cylindracea lipase gene, which were used in an attempt to amplify Malassezia lipases, yielded an amplification product after annealing at 55 degrees C only with M. pachydermatis. This specific amplification may facilitate the rapid identification of this organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.