ArticleJournal of clinical microbiology2000
A PCR-colorimetric microwell plate hybridization assay for detection of Mycobacterium tuberculosis and M. avium from culture samples and Ziehl-Neelsen-positive smears.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression.Journal of clinical microbiology · 2009Pooled it
- Systemic infection of Mycobacterium avium subspecies hominissuis and fungus in a pet dog.The Journal of veterinary medical science · 2016Article
- Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR.Journal of clinical microbiology · 2010Article
- Evaluation of molecular tools for detection and drug susceptibility testing of Mycobacterium tuberculosis in stool specimens from patients with pulmonary tuberculosis.Journal of clinical microbiology · 2010Article
- The usefulness of PCR assay in diagnosing disseminated mycobacterial infection in AIDS patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- Spoligotyping and Mycobacterium tuberculosis.Emerging infectious diseases · 2005Article
- Detection of Mycobacterium avium in blood samples of patients with AIDS by using PCR.Journal of clinical microbiology · 2002Article
- Rapid detection of Shigella species in environmental sewage by an immunocapture PCR with universal primers.Applied and environmental microbiology · 2002Article
- PCR-hybridization assay for Mycobacterium avium complex: optimization of detection in peripheral blood from humans.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiation between Mycobacterium tuberculosis and M. avium is essential for the treatment of mycobacterial infections. We have developed an easy and rapid detection assay for the diagnosis of mycobacterial diseases. This is a PCR-hybridization assay based on selective amplification of a 16S rRNA gene sequence using pan-Mycobacterium primers followed by hybridization of the amplification products to biotinylated M. tuberculosis and M. avium-specific probes. A total of 55 mycobacterial isolates were tested. For all isolates, results concordant with those of conventional identification methods were obtained. Moreover, we developed a method for extraction of DNA from Ziehl-Neelsen-positive smears which allows the recovery of intact target DNA in our PCR-hybridization assay. Our method was able to confirm all culture results for 59 Ziehl-Neelsen-positive smears from clinical specimens (35 sputum, 11 lymph node biopsy, 6 stool, 4 pus, 2 urine, and 1 pericardial fluid specimens). These data suggest that our PCR-hybridization assay, which is simple to perform and less expensive than commercial probe methods, may be suitable for the identification of M. tuberculosis and M. avium. It could become a valuable alternative approach for the diagnosis of mycobacterial infections when applied directly to DNA extracted from Ziehl-Neelsen-positive smears as well.
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