ArticleJournal of clinical microbiology2000
Identification of enterotoxigenic Escherichia coli harboring longus type IV pilus gene by DNA amplification.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- RecombinantPathogens (Basel, Switzerland) · 2023Article
- CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence.Frontiers in microbiology · 2014Article
- Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli clinical isolates from northern Colombia, South America.BioMed research international · 2014Article
- Discovery and phylogenetic analysis of novel members of class b enterotoxigenic Escherichia coli adhesive fimbriae.Journal of clinical microbiology · 2011Article
- The Longus type IV pilus of enterotoxigenic Escherichia coli (ETEC) mediates bacterial self-aggregation and protection from antimicrobial agents.Microbial pathogenesis · 2010Article
- Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins.Journal of clinical microbiology · 2009Article
- Genetic diversity of the gene cluster encoding longus, a type IV pilus of enterotoxigenic Escherichia coli.Journal of bacteriology · 2007Article
- Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse.Infection and immunity · 2005Article
- Prevalence of enterotoxigenic Escherichia coli strains harboring the longus pilus gene in Brazil.Journal of clinical microbiology · 2002Article
- Type IV longus pilus of enterotoxigenic Escherichia coli: occurrence and association with toxin types and colonization factors among strains isolated in Argentina.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA amplification of lngA, the structural gene of longus type IV pilus produced by human enterotoxigenic Escherichia coli (ETEC) was achieved by the use of specific oligonucleotide primers designed from the nucleotide sequence of lngA. A 630-bp fragment representing the entire lngA gene was amplified in eight prototype strains previously characterized as longus positive. Five ETEC strains producing colonization factor antigen III (CFA III) (also a type IV pilus) were also positive by PCR, confirming the DNA homology between CFA III and longus. None of the non-ETEC and non-E. coli enteropathogens studied showed the 0.63-kbp amplicon. The procedure thus detected only ETEC strains harboring type IV pili genes with or without other colonization factors. Except for five lngA PCR-positive, probe-positive strains, all lngA PCR-positive strains produced the pilin as demonstrated by immunoblotting. To test the amplification procedure in a clinical setting, a collection of 264 fresh clinical E. coli strains isolated from 88 Mexican children with diarrhea was screened by PCR. Among 82 ETEC isolates found, 30 (36.5%) were lngA PCR-positive. Twenty-seven percent of the children shed ETEC that possessed lngA. In parallel with DNA probes or PCR protocols to detect enterotoxin genes, the lngA PCR method may prove useful for detection of ETEC harboring type IV pilus genes in epidemiological studies.
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