ArticleApplied and environmental microbiology2000
Simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 42 citations in OpenAlex.
- An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control.Advanced pharmaceutical bulletin · 2016Article
- Spatial and temporal distribution of the vibrionaceae in coastal waters of Hawaii, Australia, and France.Microbial ecology · 2007Article
- Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification.Applied and environmental microbiology · 2005Article
- Recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.Applied and environmental microbiology · 2005Article
- New method for estimating bacterial cell abundances in natural samples by use of sublimation.Applied and environmental microbiology · 2004Article
- Two bacteria phylotypes are predominant in the Suiyo seamount hydrothermal plume.Applied and environmental microbiology · 2004Article
- Bacterial Activity at -2 to -20 degrees C in Arctic wintertime sea ice.Applied and environmental microbiology · 2004Article
- Dynamics of microbial populations and strong selection for Cycloclasticus pugetii following the Nakhodka oil spill.Microbial ecology · 2003Article
- Incidence and diversity of microorganisms within the walls of an active deep-sea sulfide chimney.Applied and environmental microbiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To rapidly and accurately enumerate total and specific microbes in aquatic samples, fluorescent in situ hybridization was combined with direct counting via direct immobilization of cells on a polymer-coated Nuclepore filter. The technique, named FISH-DC, achieved almost complete recovery of total cells and reproducibility of Psychrobacter pacificensis cells of deep-sea origin (error, </=3%) in a mixed culture and in natural seawater. Target cells immobilized on the filter were also successfully enumerated after stringent 3-cycle hybridization and even after a 16-month preservation at -30 degrees C.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.