ArticleApplied and environmental microbiology2000
Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 91 citations in OpenAlex.
- Article
- Ubiquitin-Conjugating Enzymes Ubc1 and Ubc4 Mediate the Turnover of Hap4, a Master Regulator of Mitochondrial Biogenesis inMicroorganisms · 2022Article
- Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism.eLife · 2022Article
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- Predictive models of eukaryotic transcriptional regulation reveals changes in transcription factor roles and promoter usage between metabolic conditions.Nucleic acids research · 2019Article
- Article
- Overexpression of THI4 and HAP4 Improves Glucose Metabolism and Ethanol Production inFrontiers in microbiology · 2018Article
- Fermentative metabolism impedes p53-dependent apoptosis in a Crabtree-positive but not in Crabtree-negative yeast.Journal of biosciences · 2017Article
- Increased heme synthesis in yeast induces a metabolic switch from fermentation to respiration even under conditions of glucose repression.The Journal of biological chemistry · 2017Article
- The transcription factorsMicrobial cell (Graz, Austria) · 2016Article
- GSF2 deletion increases lactic acid production by alleviating glucose repression in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Reduced Histone Expression or a Defect in Chromatin Assembly Induces Respiration.Molecular and cellular biology · 2016Article
- Overexpression of a Water-Forming NADH Oxidase Improves the Metabolism and Stress Tolerance of Saccharomyces cerevisiae in Aerobic Fermentation.Frontiers in microbiology · 2016Article
- Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.Nature communications · 2015Article
- Article
- Article
- Physiological adaptations of Saccharomyces cerevisiae evolved for improved butanol tolerance.Biotechnology for biofuels · 2013Article
- An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae.Microbial cell factories · 2012Article
- Reactive oxygen species-mediated control of mitochondrial biogenesis.International journal of cell biology · 2012Article
- Shifting the fermentative/oxidative balance in Saccharomyces cerevisiae by transcriptional deregulation of Snf1 via overexpression of the upstream activating kinase Sak1p.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.