ArticleRNA (New York, N.Y.)2000
Polyadenylation of three classes of chloroplast RNA in Chlamydomonas reinhadtii.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 80 citations in OpenAlex.
- Comparative Transcriptome Profiling ofPlants (Basel, Switzerland) · 2021Article
- Identification of polycistronic transcriptional units and non-canonical introns in green algal chloroplasts based on long-read RNA sequencing data.BMC genomics · 2021Article
- Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.Nucleic acids research · 2018Article
- High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates.The Plant journal : for cell and molecular biology · 2018Article
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- Characterization of the Role of Hexamer AGUAAA and Poly(A) Tail in Coronavirus Polyadenylation.PloS one · 2016Article
- Experimental Genome-Wide Determination of RNA Polyadenylation in Chlamydomonas reinhardtii.PloS one · 2016Article
- Regulation of coronaviral poly(A) tail length during infection is not coronavirus species- or host cell-specific.Virus genes · 2014Article
- Article
- Overaccumulation of the chloroplast antisense RNA AS5 is correlated with decreased abundance of 5S rRNA in vivo and inefficient 5S rRNA maturation in vitro.RNA (New York, N.Y.) · 2011Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Article
- The nucleus-encoded factor MCD4 participates in degradation of nonfunctional 3' UTR sequences generated by cleavage of pre-mRNA in Chlamydomonas chloroplasts.Molecular genetics and genomics : MGG · 2007Article
- Polyadenylation of a functional mRNA controls gene expression in Escherichia coli.Nucleic acids research · 2007Article
- Antisense transcript and RNA processing alterations suppress instability of polyadenylated mRNA in chlamydomonas chloroplasts.The Plant cell · 2004Article
- Chloroplast RNA processing and stability.Photosynthesis research · 2004Article
- Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.Nucleic acids research · 2004Article
- AtmtPNPase is required for multiple aspects of the 18S rRNA metabolism in Arabidopsis thaliana mitochondria.Nucleic acids research · 2004Article
- Domain analysis of the chloroplast polynucleotide phosphorylase reveals discrete functions in RNA degradation, polyadenylation, and sequence homology with exosome proteins.The Plant cell · 2003Article
- The Chlamydomonas reinhardtii organellar genomes respond transcriptionally and post-transcriptionally to abiotic stimuli.The Plant cell · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three classes of RNA, represented by atpB and petD mRNAs, Arg and Glu tRNAs, and 5S rRNA, were found to exist in polyadenylated form in Chlamydomonas reinhardtii chloroplasts. Sequence analysis of cDNA clones derived from reverse transcriptase-polymerase chain reaction protocols used to select polyadenylated RNAs revealed that, at least for the mRNAs and tRNAs, there are three apparent types of polyadenylation. In the first case, the poly(A) tail is added at or near the mature 3' end, even when this follows a strong secondary structure. In the second case, the tail is added to pre-mRNA or pre-tRNA, suggesting a possible competition between polyadenylation and RNA-processing pathways. Finally, in all cases, the poly(A) tail can be added internally, possibly as a part of an RNA-decay pathway. The tails found in Chlamydomonas chloroplasts differ from those of spinach chloroplasts in adenine content, being nearly homopolymeric (>98% adenine) versus 70% in spinach, and are similar in length to those of Escherichia coli, being mostly between 20 and 50 nt. In vitro assays using a Chlamydomonas chloroplast protein extract showed that a 3' end A25 tail was sufficient to stimulate rapid degradation of atpB RNA in vitro, with a lesser effect for petD, and only minor effects on trnE. We therefore propose that polyadenylation contributes to mRNA degradation in Chlamydomonas chloroplasts, but that its effect may vary.
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