ArticleGenes & development2000
Nuclear localization and histone acetylation: a pathway for chromatin opening and transcriptional activation of the human beta-globin locus.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 308 citations in OpenAlex.
- Mechanisms of Globin Gene Regulation in Mammals.Annual review of genetics · 2025Review
- KMT2C/KMT2D-dependent H3K4me1 mediates changes in DNA replication timing and origin activity during a cell fate transition.Cell reports · 2025Article
- Guiding the HBO1 complex function through the JADE subunit.Nature structural & molecular biology · 2024Article
- MLL3/MLL4 enzymatic activity shapes DNA replication timing.bioRxiv : the preprint server for biology · 2023Article
- Review
- 3D reconstruction of genomic regions from sparse interaction data.NAR genomics and bioinformatics · 2021Article
- Nuclear Mechanotransduction in Skeletal Muscle.Cells · 2021Review
- Developmental differences in genome replication program and origin activation.Nucleic acids research · 2020Article
- Review
- Control of DNA replication timing in the 3D genome.Nature reviews. Molecular cell biology · 2019Review
- Review
- Article
- An intergenic non-coding RNA promoter required for histone modifications in the human β-globin chromatin domain.PloS one · 2019Article
- Cell-Specific PEAR1 Methylation Studies Reveal a Locus that Coordinates Expression of Multiple Genes.International journal of molecular sciences · 2018Article
- Ethylene induces combinatorial effects of histone H3 acetylation in gene expression in Arabidopsis.BMC genomics · 2017Article
- The Nuclear Option: Evidence Implicating the Cell Nucleus in Mechanotransduction.Journal of biomechanical engineering · 2017Review
- 3D genomics imposes evolution of the domain model of eukaryotic genome organization.Chromosoma · 2017Review
- In the loop: promoter-enhancer interactions and bioinformatics.Briefings in bioinformatics · 2016Review
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- The Hematopoietic Stem and Progenitor Cell Cistrome: GATA Factor-Dependent cis-Regulatory Mechanisms.Current topics in developmental biology · 2016Review
70 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
We have investigated the mechanism, structural correlates, and cis-acting elements involved in chromatin opening and gene activation, using the human beta-globin locus as a model. Full transcriptional activity of the human beta-globin locus requires the locus control region (LCR), composed of a series of nuclease hypersensitive sites located upstream of this globin gene cluster. Our previous analysis of naturally occurring and targeted LCR deletions revealed that chromatin opening and transcriptional activity in the endogenous beta-globin locus are dissociable and dependent on distinct cis-acting elements. We now report that general histone H3/H4 acetylation and relocation of the locus away from centromeric heterochromatin in the interphase nucleus are correlated and do not require the LCR. In contrast, LCR-dependent promoter activation is associated with localized histone H3 hyperacetylation at the LCR and the transcribed beta-globin-promoter and gene. On the basis of these results, we suggest a multistep model for gene activation; localization away from centromeric heterochromatin is required to achieve general hyperacetylation and an open chromatin structure of the locus, whereas a mechanism involving LCR/promoter histone H3 hyperacetylation is required for high-level transcription of the beta-globin genes.
Indexed as
Identifiers
10783166PMC316536W2140475749What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.