ArticleJournal of bacteriology2000
Growth phase-coupled changes of the ribosome profile in natural isolates and laboratory strains of Escherichia coli.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 68 citations in OpenAlex.
- Role of starvation survival response mechanisms on ribosome integrity, antibiotic tolerances, and virulence of Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2026Article
- Ribosome Protein Composition Mediates Translation during theInternational journal of molecular sciences · 2023Article
- Absence of Ribosome Modulation Factor Alters Growth and Competitive Fitness of Escherichia coli.Microbiology spectrum · 2022Article
- Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides.iScience · 2021Article
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- Transcription regulates ribosome hibernation.Molecular microbiology · 2021Article
- Functional Characterization of the Pseudomonas aeruginosa Ribosome Hibernation-Promoting Factor.Journal of bacteriology · 2020Article
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- Engineering the Translational Machinery for Biotechnology Applications.Molecular biotechnology · 2020Review
- RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.Nucleic acids research · 2020Article
- Disassembly of theProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structure of theThe EMBO journal · 2017Article
- Resuscitation ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The stringent response regulates adaptation to darkness in the cyanobacterium Synechococcus elongatus.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ribosome hibernation factor promotes Staphylococcal survival and differentially represses translation.Nucleic acids research · 2016Article
- Bacteriophage T4 Infection of Stationary Phase E. coli: Life after Log from a Phage Perspective.Frontiers in microbiology · 2016Article
- Ribosome hibernation facilitates tolerance of stationary-phase bacteria to aminoglycosides.Antimicrobial agents and chemotherapy · 2015Article
- Identification of genes essential for pellicle formation in Acinetobacter baumannii.BMC microbiology · 2015Article
- The Listeria monocytogenes hibernation-promoting factor is required for the formation of 100S ribosomes, optimal fitness, and pathogenesis.Journal of bacteriology · 2015Article
- New approaches to the problem of generating coherent, reproducible phenotypes.Theory in biosciences = Theorie in den Biowissenschaften · 2014Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growth phase-dependent change in sucrose density gradient centrifugation patterns of ribosomes was analyzed for both laboratory strains of Escherichia coli and natural isolates from the ECOR collection. All of the natural isolates examined formed 100S ribosome dimers in the stationary phase, and ribosome modulation factor (RMF) was associated with the ribosome dimers in the ECOR strains as in the laboratory strain W3110. The ribosome profile (70S monomers versus 100S dimers) follows a defined pattern over time during lengthy culture in both the laboratory strains and natural isolates. There are four discrete stages: (i) formation of 100S dimers in the early stationary phase; (ii) transient decrease in the dimer level; (iii) return of dimers to the maximum level; and (iv) dissociation of 100S dimers into 70S ribosomes, which are quickly degraded into subassemblies. The total time for this cycle of ribosome profile change, however, varied from strain to strain, resulting in apparent differences in the ribosome profiles when observed at a fixed time point. A correlation was noted in all strains between the decay of 100S ribosomes and the subsequent loss of cell viability. Two types of E. coli mutants defective in ribosome dimerization were identified, both of which were unable to survive for a prolonged period in stationary phase. The W3110 mutant, with a disrupted rmf gene, has a defect in ribosome dimerization because of lack of RMF, while strain Q13 is unable to form ribosome dimers due to a ribosomal defect in binding RMF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.