ArticleJournal of bacteriology2000
Catabolism of alpha-ketoglutarate by a sucA mutant of Bradyrhizobium japonicum: evidence for an alternative tricarboxylic acid cycle.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 79 citations in OpenAlex.
- Host-dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by diazotrophic Actinomycetota Frankia cluster-2.Journal of experimental botany · 2025Article
- Pleiotropic Effects of PhaR Regulator inInternational journal of molecular sciences · 2024Article
- Biochemical and Structural Insights into a Thiamine Diphosphate-Dependent α-Ketoglutarate Decarboxylase from CyanobacteriumInternational journal of molecular sciences · 2023Article
- Production of selenium nanoparticles occurs through an interconnected pathway of sulphur metabolism and oxidative stress response in Pseudomonas putida KT2440.Microbial biotechnology · 2023Article
- Genetic variation in symbiotic islands of natural variant strains of soybeanMicrobial genomics · 2022Article
- The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.Signal transduction and targeted therapy · 2021Article
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- Nitrogen Assimilation and Transport byInternational journal of molecular sciences · 2020Article
- A Uniquely Complex Mitochondrial Proteome from Euglena gracilis.Molecular biology and evolution · 2020Article
- Kinetic and structural insights into enzymatic mechanism of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142.PloS one · 2020Article
- Energy metabolism in anaerobic eukaryotes and Earth's late oxygenation.Free radical biology & medicine · 2019Review
- Isocitrate Lyase and Succinate Semialdehyde Dehydrogenase Mediate the Synthesis of α-Ketoglutarate inFrontiers in microbiology · 2019Article
- An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation inFrontiers in microbiology · 2019Article
- Proteomic Characterization ofInternational journal of molecular sciences · 2018Article
- Concomitant Loss of the Glyoxalase System and Glycolysis Makes the Uncultured Pathogen "Candidatus Liberibacter asiaticus" an Energy Scavenger.Applied and environmental microbiology · 2017Article
- Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens.Applied and environmental microbiology · 2016Article
- Natural and Synthetic Variants of the Tricarboxylic Acid Cycle in Cyanobacteria: Introduction of the GABA Shunt intoFrontiers in microbiology · 2016Article
- Article
- Biochemistry and evolution of anaerobic energy metabolism in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2012Review
- Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A complete tricarboxylic acid (TCA) cycle is generally considered necessary for energy production from the dicarboxylic acid substrates malate, succinate, and fumarate. However, a Bradyrhizobium japonicum sucA mutant that is missing alpha-ketoglutarate dehydrogenase is able to grow on malate as its sole source of carbon. This mutant also fixes nitrogen in symbiosis with soybean, where dicarboxylic acids are its principal carbon substrate. Using a flow chamber system to make direct measurements of oxygen consumption and ammonium excretion, we confirmed that bacteroids formed by the sucA mutant displayed wild-type rates of respiration and nitrogen fixation. Despite the absence of alpha-ketoglutarate dehydrogenase activity, whole cells of the mutant were able to decarboxylate alpha-[U-(14)C]ketoglutarate and [U-(14)C]glutamate at rates similar to those of wild-type B. japonicum, indicating that there was an alternative route for alpha-ketoglutarate catabolism. Because cell extracts from B. japonicum decarboxylated [U-(14)C]glutamate very slowly, the gamma-aminobutyrate shunt is unlikely to be the pathway responsible for alpha-ketoglutarate catabolism in the mutant. In contrast, cell extracts from both the wild type and mutant showed a coenzyme A (CoA)-independent alpha-ketoglutarate decarboxylation activity. This activity was independent of pyridine nucleotides and was stimulated by thiamine PP(i). Thin-layer chromatography showed that the product of alpha-ketoglutarate decarboxylation was succinic semialdehyde. The CoA-independent alpha-ketoglutarate decarboxylase, along with succinate semialdehyde dehydrogenase, may form an alternative pathway for alpha-ketoglutarate catabolism, and this pathway may enhance TCA cycle function during symbiotic nitrogen fixation.
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