ArticleJournal of bacteriology2000
Expression of uptake hydrogenase and molybdenum nitrogenase in Rhodobacter capsulatus is coregulated by the RegB-RegA two-component regulatory system.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 106 citations in OpenAlex.
- Atmospheric hydrogen consumption is regulated by glycerol-mediated catabolite repression in mycobacteria.mSystems · 2026Article
- Transcription of Nitrogen Fixation Genes Is Enhanced at Unfavorably High Oxygen Concentrations for Diazotrophic Growth in a Methane-oxidizing Bacterium.Microbes and environments · 2025Article
- Integrated genomics provides insights into the evolution of the polyphosphate accumulation trait ofEnvironmental science and ecotechnology · 2024Article
- Article
- A directed genome evolution method to enhance hydrogen production inFrontiers in microbiology · 2022Article
- TCA Cycle Replenishing Pathways in Photosynthetic Purple Non-Sulfur Bacteria Growing with Acetate.Life (Basel, Switzerland) · 2021Review
- NifA is the master regulator of both nitrogenase systems in Rhodobacter capsulatus.MicrobiologyOpen · 2019Article
- Transcriptomic analysis of aerobic respiratory and anaerobic photosynthetic states inMicrobial genomics · 2017Article
- The Global Redox Responding RegB/RegA Signal Transduction System Regulates the Genes Involved in Ferrous Iron and Inorganic Sulfur Compound Oxidation of the AcidophilicFrontiers in microbiology · 2017Article
- The RegA regulon exhibits variability in response to altered growth conditions and differs markedly betweenMicrobial genomics · 2016Article
- Oxidation of Molecular Hydrogen by a Chemolithoautotrophic Beggiatoa Strain.Applied and environmental microbiology · 2016Article
- Transcriptional regulation of the Rhodobacter capsulatus response regulator CtrA.Microbiology (Reading, England) · 2013Article
- Biogenesis of cbb(3)-type cytochrome c oxidase in Rhodobacter capsulatus.Biochimica et biophysica acta · 2012Review
- The poor growth of Rhodospirillum rubrum mutants lacking RubisCO is due to the accumulation of ribulose-1,5-bisphosphate.Journal of bacteriology · 2011Article
- Characterization of the hupSL promoter activity in Nostoc punctiforme ATCC 29133.BMC microbiology · 2009Article
- Role of the global transcriptional regulator PrrA in Rhodobacter sphaeroides 2.4.1: combined transcriptome and proteome analysis.Journal of bacteriology · 2008Article
- Purification and assays of Rhodobacter capsulatus RegB-RegA two-component signal transduction system.Methods in enzymology · 2007Article
- Regulation of uptake hydrogenase and effects of hydrogen utilization on gene expression in Rhodopseudomonas palustris.Journal of bacteriology · 2006Article
- Activation of the global gene regulator PrrA (RegA) from Rhodobacter sphaeroides.Biochemistry · 2006Article
- Identification of a ubiquinone-binding site that affects autophosphorylation of the sensor kinase RegB.The Journal of biological chemistry · 2006Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Purple photosynthetic bacteria are capable of generating cellular energy from several sources, including photosynthesis, respiration, and H(2) oxidation. Under nutrient-limiting conditions, cellular energy can be used to assimilate carbon and nitrogen. This study provides the first evidence of a molecular link for the coregulation of nitrogenase and hydrogenase biosynthesis in an anoxygenic photosynthetic bacterium. We demonstrated that molybdenum nitrogenase biosynthesis is under the control of the RegB-RegA two-component regulatory system in Rhodobacter capsulatus. Footprint analyses and in vivo transcription studies showed that RegA indirectly activates nitrogenase synthesis by binding to and activating the expression of nifA2, which encodes one of the two functional copies of the nif-specific transcriptional activator, NifA. Expression of nifA2 but not nifA1 is reduced in the reg mutants up to eightfold under derepressing conditions and is also reduced under repressing conditions. Thus, although NtrC is absolutely required for nifA2 expression, RegA acts as a coactivator of nifA2. We also demonstrated that in reg mutants, [NiFe]hydrogenase synthesis and activity are increased up to sixfold. RegA binds to the promoter of the hydrogenase gene operon and therefore directly represses its expression. Thus, the RegB-RegA system controls such diverse processes as energy-generating photosynthesis and H(2) oxidation, as well as the energy-demanding processes of N(2) fixation and CO(2) assimilation.
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