ArticleJournal of bacteriology2000
Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 240 citations in OpenAlex.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- A novel reverse lipase toxin substrate of themicroLife · 2026Article
- New Yarrowia lipolytica chassis strains for industrial enzyme production.Microbial cell factories · 2025Article
- FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides.Applied microbiology and biotechnology · 2024Article
- Article
- Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system.FEMS yeast research · 2023Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Application of a New Engineered Strain ofInternational journal of molecular sciences · 2021Article
- Studies on the Catalytic Properties of Crude Freeze-Dried Preparations ofBiomolecules · 2021Article
- Genomic analysis of novel Yarrowia-like yeast symbionts associated with the carrion-feeding burying beetle Nicrophorus vespilloides.BMC genomics · 2021Article
- Phylogenomic and biochemical analysis reassesses temperate marine yeast Yarrowia lipolytica NCIM 3590 to be Yarrowia bubula.Scientific reports · 2021Article
- Application of freeze-dried Yarrowia lipolytica biomass in the synthesis of lipophilic antioxidants.Biotechnology letters · 2021Article
- Metabolic engineering of Saccharomyces cerevisiae for production of β-carotene from hydrophobic substrates.FEMS yeast research · 2021Article
- Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.Journal of industrial microbiology & biotechnology · 2020Review
- Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous HBiotechnology for biofuels · 2020Article
- High-Level Production of a Thermostable Mutant ofInternational journal of molecular sciences · 2019Article
- Optimization of physical parameters for enhanced production of lipase from Staphylococcus hominis using response surface methodology.Environmental science and pollution research international · 2019Article
- Noncovalent Immobilization ofBiomolecules · 2019Article
- Cellular and metabolic engineering of oleaginous yeastEngineering in life sciences · 2019Review
- Yarrowia lipolytica: a beneficious yeast in biotechnology as a rare opportunistic fungal pathogen: a minireview.World journal of microbiology & biotechnology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated the LIP2 gene from the lipolytic yeast Yarrowia lipolytica. It was found to encode a 334-amino-acid precursor protein. The secreted lipase is a 301-amino-acid glycosylated polypeptide which is a member of the triacylglycerol hydrolase family (EC 3.1.1.3). The Lip2p precursor protein is processed by the KEX2-like endoprotease encoded by XPR6. Deletion of the XPR6 gene resulted in the secretion of an active but less stable proenzyme. Thus, the pro region does not inhibit lipase secretion and activity. However, it does play an essential role in the production of a stable enzyme. Processing was found to be correct in LIP2(A) (multiple LIP2 copy integrant)-overexpressing strains, which secreted 100 times more activity than the wild type, demonstrating that XPR6 maturation was not limiting. No extracellular lipase activity was detected with the lip2 knockout (KO) strain, strongly suggesting that extracellular lipase activity results from expression of the LIP2 gene. Nevertheless, the lip2 KO strain is still able to grow on triglycerides, suggesting an alternative pathway for triglyceride utilization in Y. lipolytica.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.