ArticleJournal of bacteriology2000
Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552'phoA in vitro transposition system.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Predominantly Orphan Secretome in the Lung PathogenMicroorganisms · 2024Article
- In silico and in vitro study of Mycobacterium tuberculosis H37Rv uncharacterized protein (RipD): an insight on tuberculosis therapeutics.Journal of molecular modeling · 2022Article
- Multiple acyl-CoA dehydrogenase deficiency kills Mycobacterium tuberculosis in vitro and during infection.Nature communications · 2021Article
- Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation.ACS infectious diseases · 2018Article
- Green Fluorescent Protein as a protein localization and topological reporter in mycobacteria.Tuberculosis (Edinburgh, Scotland) · 2017Article
- Separable roles for Mycobacterium tuberculosis ESX-3 effectors in iron acquisition and virulence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Rip1 protease of Mycobacterium tuberculosis controls the SigD regulon.Journal of bacteriology · 2014Article
- Site-2 protease substrate specificity and coupling in trans by a PDZ-substrate adapter protein.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Borrelia burgdorferi HtrA may promote dissemination and irritation.Molecular microbiology · 2013Article
- Article
- Substrate specificity of MarP, a periplasmic protease required for resistance to acid and oxidative stress in Mycobacterium tuberculosis.The Journal of biological chemistry · 2013Article
- Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras.BMC infectious diseases · 2013Article
- Virulence factors of the Mycobacterium tuberculosis complex.Virulence · 2013Review
- Enhanced priming of adaptive immunity by Mycobacterium smegmatis mutants with high-level protein secretion.Clinical and vaccine immunology : CVI · 2012Article
- Inhibition of the sole type I signal peptidase of Mycobacterium tuberculosis is bactericidal under replicating and nonreplicating conditions.Journal of bacteriology · 2012Article
- Genome-wide identification of Mycobacterium tuberculosis exported proteins with roles in intracellular growth.Journal of bacteriology · 2011Article
- Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria.The Journal of biological chemistry · 2008Article
- Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria.Molecular microbiology · 2008Article
- Species of environmental mycobacteria differ in their abilities to grow in human, mouse, and carp macrophages and with regard to the presence of mycobacterial virulence genes, as observed by DNA microarray hybridization.Applied and environmental microbiology · 2008Article
- Beta-lactamase can function as a reporter of bacterial protein export during Mycobacterium tuberculosis infection of host cells.Microbiology (Reading, England) · 2007Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the transposon inserts randomly into target DNA. M. tuberculosis genomic libraries were targeted with Tn552'phoA transposons, and these libraries were screened in M. smegmatis for active PhoA translational fusions. Thirty-two different M. tuberculosis open reading frames were identified; eight contain standard signal peptides, six contain lipoprotein signal peptides, and seventeen contain one or more transmembrane domains. Four of these proteins had not yet been assigned as exported proteins in the M. tuberculosis databases. This collection of exported proteins includes factors that are known to participate in the immune response of M. tuberculosis and proteins with homologies, suggesting a role in pathogenesis. Nine of the proteins appear to be unique to mycobacteria and represent promising candidates for factors that participate in protective immunity and virulence. This technology of creating comprehensive fusion libraries should be applicable to other organisms.
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