ArticleJournal of bacteriology2000
Haloalkane-utilizing Rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 68 citations in OpenAlex.
- The aldehyde dehydrogenase superfamilies: correlations and deviations in structure and function.Applied microbiology and biotechnology · 2025Review
- Norwegian Soils and Waters Contain Mesophilic, Plastic-Degrading Bacteria.Microorganisms · 2021Article
- Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA.Current microbiology · 2019Article
- A Pseudomonas putida strain genetically engineered for 1,2,3-trichloropropane bioremediation.Applied and environmental microbiology · 2014Article
- Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Transformation and biodegradation of 1,2,3-trichloropropane (TCP).Environmental science and pollution research international · 2012Review
- Facilitation of bacterial adaptation to chlorothalonil-contaminated sites by horizontal transfer of the chlorothalonil hydrolytic dehalogenase gene.Applied and environmental microbiology · 2011Article
- Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23.Extremophiles : life under extreme conditions · 2010Article
- Biodegradation of chlorinated alkanes and their commercial mixtures by Pseudomonas sp. strain 273.Journal of industrial microbiology & biotechnology · 2006Article
- Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases.Applied and environmental microbiology · 2005Article
- Distribution of the coenzyme M pathway of epoxide metabolism among ethene- and vinyl chloride-degrading Mycobacterium strains.Applied and environmental microbiology · 2003Article
- Diversity of nitrile hydratase and amidase enzyme genes in Rhodococcus erythropolis recovered from geographically distinct habitats.Applied and environmental microbiology · 2003Article
- Biodegradation, biotransformation, and biocatalysis (b3).Applied and environmental microbiology · 2002Review
- Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium avium N85 and preliminary characterization of DhmA.Applied and environmental microbiology · 2002Article
- Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene.Applied and environmental microbiology · 2002Article
- Functionally relevant motions of haloalkane dehalogenases occur in the specificity-modulating cap domains.Protein science : a publication of the Protein Society · 2002Article
- Genetic characterization and evolutionary implications of a car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10.Journal of bacteriology · 2001Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sequences of the 16S rRNA and haloalkane dehalogenase (dhaA) genes of five gram-positive haloalkane-utilizing bacteria isolated from contaminated sites in Europe, Japan, and the United States and of the archetypal haloalkane-degrading bacterium Rhodococcus sp. strain NCIMB13064 were compared. The 16S rRNA gene sequences showed less than 1% sequence divergence, and all haloalkane degraders clearly belonged to the genus Rhodococcus. All strains shared a completely conserved dhaA gene, suggesting that the dhaA genes were recently derived from a common ancestor. The genetic organization of the dhaA gene region in each of the haloalkane degraders was examined by hybridization analysis and DNA sequencing. Three different groups could be defined on the basis of the extent of the conserved dhaA segment. The minimal structure present in all strains consisted of a conserved region of 12.5 kb, which included the haloalkane-degradative gene cluster that was previously found in strain NCIMB13064. Plasmids of different sizes were found in all strains. Southern hybridization analysis with a dhaA gene probe suggested that all haloalkane degraders carry the dhaA gene region both on the chromosome and on a plasmid (70 to 100 kb). This suggests that an ancestral plasmid was transferred between these Rhodococcus strains and subsequently has undergone insertions or deletions. In addition, transposition events and/or plasmid integration may be responsible for positioning the dhaA gene region on the chromosome. The data suggest that the haloalkane dehalogenase gene regions of these gram-positive haloalkane-utilizing bacteria are composed of a single catabolic gene cluster that was recently distributed worldwide.
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